Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells...
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Taylor & Francis
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34639/ |
| _version_ | 1848847829666103296 |
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| author | Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Tajuddin, Saiful Nizam |
| author_facet | Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Tajuddin, Saiful Nizam |
| author_sort | Ismail, Norsharina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1–42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aβ1–42-induced cell death. Flavonoids (3–6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. |
| first_indexed | 2025-11-15T09:24:50Z |
| format | Article |
| id | upm-34639 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:24:50Z |
| publishDate | 2015 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-346392015-12-16T07:46:32Z http://psasir.upm.edu.my/id/eprint/34639/ Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Tajuddin, Saiful Nizam The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1–42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aβ1–42-induced cell death. Flavonoids (3–6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. Taylor & Francis 2015 Article PeerReviewed Ismail, Norsharina and Akhtar, Muhammad Nadeem and Ismail, Maznah and Zareen, Seema and Shah, Syed Adnan Ali and Lajis, Nordin and Tajuddin, Saiful Nizam (2015) Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells. Natural Product Research, 29 (16). pp. 1571-1574. ISSN 1478-6419; ESSN: 1478-6427 http://www.tandfonline.com/doi/full/10.1080/14786419.2014.985676 10.1080/14786419.2014.985676 |
| spellingShingle | Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Tajuddin, Saiful Nizam Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title | Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title_full | Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title_fullStr | Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title_full_unstemmed | Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title_short | Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells |
| title_sort | neuroprotective effect from stem bark extracts of knema laurina against h₂o₂- and aβ(1-42)-induced cell death in human sh-sy5y cells |
| url | http://psasir.upm.edu.my/id/eprint/34639/ http://psasir.upm.edu.my/id/eprint/34639/ http://psasir.upm.edu.my/id/eprint/34639/ |