Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population...
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| Format: | Article |
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Penerbit Universiti Sains Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34638/ |
| _version_ | 1848847829413396480 |
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| author | Liyang, Gao Abdullah, Syahrilnizam Rosli, Rozita Nordin, Norshariza |
| author_facet | Liyang, Gao Abdullah, Syahrilnizam Rosli, Rozita Nordin, Norshariza |
| author_sort | Liyang, Gao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population of functional neurons. So far, a number of protocols have been established to promote the differentiation of ESCs, such as direct and indirect differentiation. One of the widely used protocols to generate neurons is through the spontaneous formation of multicellular aggregates known as embryonic bodies (EBs). However, for some, it is not clear why EB protocol could be the protocol of choice. EB also is known to mimic an early embryo; hence, knowing the similarities between EB and an early embryo is essential, particularly the information on the players that promote the formation of EBs or the aggregation of ESCs. This review paper focuses on these issues and discusses further the generation of neural cells from EBs using a well-known protocol, the 4-/4+ protocol. |
| first_indexed | 2025-11-15T09:24:49Z |
| format | Article |
| id | upm-34638 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:24:49Z |
| publishDate | 2014 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-346382015-12-16T07:42:19Z http://psasir.upm.edu.my/id/eprint/34638/ Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) Liyang, Gao Abdullah, Syahrilnizam Rosli, Rozita Nordin, Norshariza An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population of functional neurons. So far, a number of protocols have been established to promote the differentiation of ESCs, such as direct and indirect differentiation. One of the widely used protocols to generate neurons is through the spontaneous formation of multicellular aggregates known as embryonic bodies (EBs). However, for some, it is not clear why EB protocol could be the protocol of choice. EB also is known to mimic an early embryo; hence, knowing the similarities between EB and an early embryo is essential, particularly the information on the players that promote the formation of EBs or the aggregation of ESCs. This review paper focuses on these issues and discusses further the generation of neural cells from EBs using a well-known protocol, the 4-/4+ protocol. Penerbit Universiti Sains Malaysia 2014 Article PeerReviewed Liyang, Gao and Abdullah, Syahrilnizam and Rosli, Rozita and Nordin, Norshariza (2014) Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs). Malaysian Journal of Medical Sciences, 21 (5). pp. 8-16. ISSN 1394-195X; ESSN: 2180-4303 http://www.mjms.usm.my/default.asp?tag=14&kod_volume=77 |
| spellingShingle | Liyang, Gao Abdullah, Syahrilnizam Rosli, Rozita Nordin, Norshariza Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title | Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title_full | Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title_fullStr | Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title_full_unstemmed | Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title_short | Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) |
| title_sort | neural commitment of embryonic stem cells through the formation of embryoid bodies (ebs) |
| url | http://psasir.upm.edu.my/id/eprint/34638/ http://psasir.upm.edu.my/id/eprint/34638/ |