Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia
In this study the genetic diversity of local freshwater leeches (Hirudinaria spp.) was inferred using mtDNA COI gene analysis and compared with the gross external variations of 26 freshwater leech specimens obtained from the wild and leech farms. Based on a neighbor-joining tree generated from 516 C...
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| Format: | Article |
| Language: | English |
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Springer
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34600/ http://psasir.upm.edu.my/id/eprint/34600/1/Morphological%20and%20genetic%20variations%20of%20the%20freshwater%20leech%2C%20Hirudinaria%20spp.%2C%20in%20Peninsular%20Malaysia.pdf |
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| author | Chong, Lee Kim Ong, Alan Han Kiat Tan, Soon Guan Taranjeet, K. A. S. Peris, M. M. Sana, A. M. M. A. Hassan, H. R. |
| author_facet | Chong, Lee Kim Ong, Alan Han Kiat Tan, Soon Guan Taranjeet, K. A. S. Peris, M. M. Sana, A. M. M. A. Hassan, H. R. |
| author_sort | Chong, Lee Kim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study the genetic diversity of local freshwater leeches (Hirudinaria spp.) was inferred using mtDNA COI gene analysis and compared with the gross external variations of 26 freshwater leech specimens obtained from the wild and leech farms. Based on a neighbor-joining tree generated from 516 COI base sequences, four distinct clades of Hirudinaria were seen with interspecific genetic divergence in the range of 7.6–14.5%. The external morphological variations based on the presence of stripes, location of gonopores, and anus separated the samples into four morphologically distinct groups matching the four clades obtained from the molecular data. Two black stripes at the ventral region were observed only in specimens found clustered with clades that contained the GenBank-reported H. manillensis, whereas the brown or dark green coloration without stripes on the ventral region was seen in samples that clustered with H. javanica and H. bpling clades. |
| first_indexed | 2025-11-15T09:24:40Z |
| format | Article |
| id | upm-34600 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:24:40Z |
| publishDate | 2014 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-346002016-09-19T05:07:56Z http://psasir.upm.edu.my/id/eprint/34600/ Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia Chong, Lee Kim Ong, Alan Han Kiat Tan, Soon Guan Taranjeet, K. A. S. Peris, M. M. Sana, A. M. M. A. Hassan, H. R. In this study the genetic diversity of local freshwater leeches (Hirudinaria spp.) was inferred using mtDNA COI gene analysis and compared with the gross external variations of 26 freshwater leech specimens obtained from the wild and leech farms. Based on a neighbor-joining tree generated from 516 COI base sequences, four distinct clades of Hirudinaria were seen with interspecific genetic divergence in the range of 7.6–14.5%. The external morphological variations based on the presence of stripes, location of gonopores, and anus separated the samples into four morphologically distinct groups matching the four clades obtained from the molecular data. Two black stripes at the ventral region were observed only in specimens found clustered with clades that contained the GenBank-reported H. manillensis, whereas the brown or dark green coloration without stripes on the ventral region was seen in samples that clustered with H. javanica and H. bpling clades. Springer 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34600/1/Morphological%20and%20genetic%20variations%20of%20the%20freshwater%20leech%2C%20Hirudinaria%20spp.%2C%20in%20Peninsular%20Malaysia.pdf Chong, Lee Kim and Ong, Alan Han Kiat and Tan, Soon Guan and Taranjeet, K. A. S. and Peris, M. M. and Sana, A. M. M. A. and Hassan, H. R. (2014) Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia. Biochemical Genetics, 52 (5-6). pp. 283-295. ISSN 0006-2928; ESSN: 1573-4927 http://link.springer.com/article/10.1007/s10528-014-9647-8?view=classic 10.1007/s10528-014-9647-8 |
| spellingShingle | Chong, Lee Kim Ong, Alan Han Kiat Tan, Soon Guan Taranjeet, K. A. S. Peris, M. M. Sana, A. M. M. A. Hassan, H. R. Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title | Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title_full | Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title_fullStr | Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title_full_unstemmed | Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title_short | Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia |
| title_sort | morphological and genetic variations of the freshwater leech, hirudinaria spp., in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/34600/ http://psasir.upm.edu.my/id/eprint/34600/ http://psasir.upm.edu.my/id/eprint/34600/ http://psasir.upm.edu.my/id/eprint/34600/1/Morphological%20and%20genetic%20variations%20of%20the%20freshwater%20leech%2C%20Hirudinaria%20spp.%2C%20in%20Peninsular%20Malaysia.pdf |