A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (I...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3460/ http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf |
| _version_ | 1848839536412459008 |
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| author | Thiruvengadam, Muthu Jayabalan, Narayanasamypillai |
| author_facet | Thiruvengadam, Muthu Jayabalan, Narayanasamypillai |
| author_sort | Thiruvengadam, Muthu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were
successfully transferred to fields. |
| first_indexed | 2025-11-15T07:13:00Z |
| format | Article |
| id | upm-3460 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:13:00Z |
| publishDate | 2003 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-34602015-09-11T02:54:56Z http://psasir.upm.edu.my/id/eprint/3460/ A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) Thiruvengadam, Muthu Jayabalan, Narayanasamypillai An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields. Universiti Putra Malaysia Press 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf Thiruvengadam, Muthu and Jayabalan, Narayanasamypillai (2003) A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain). Pertanika Journal of Tropical Agricultural Science, 26 (1). pp. 35-40. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2026%20(1)%20Apr.%202003/06%20JTAS%20Vol.26%20(1)%202003%20(Pg%2035-40).pdf |
| spellingShingle | Thiruvengadam, Muthu Jayabalan, Narayanasamypillai A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title | A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title_full | A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title_fullStr | A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title_full_unstemmed | A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title_short | A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) |
| title_sort | protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (lablab purpureus var. lignosus (l) prain) |
| url | http://psasir.upm.edu.my/id/eprint/3460/ http://psasir.upm.edu.my/id/eprint/3460/ http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf |