Molecular dynamics of thermoenzymes at high temperature and pressure: a review
Lipases are known for their versatility in addition to their ability to digest fat. They can be used for the formulation of detergents, as food ingredients and as biocatalysts in many industrial processes. Because conventional enzymes are frangible at high temperatures, the replacement of convention...
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| Format: | Article |
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Springer
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34582/ |
| _version_ | 1848847814066438144 |
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| author | Karjiban, Roghayeh Abedi Lim, Wui Zhuan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin |
| author_facet | Karjiban, Roghayeh Abedi Lim, Wui Zhuan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin |
| author_sort | Karjiban, Roghayeh Abedi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipases are known for their versatility in addition to their ability to digest fat. They can be used for the formulation of detergents, as food ingredients and as biocatalysts in many industrial processes. Because conventional enzymes are frangible at high temperatures, the replacement of conventional chemical routes with biochemical processes that utilize thermostable lipases is vital in the industrial setting. Recent theoretical studies on enzymes have provided numerous fundamental insights into the structures, folding mechanisms and stabilities of these proteins. The studies corroborate the experimental results and provide additional information regarding the structures that were determined experimentally. In this paper, we review the computational studies that have described how temperature affects the structure and dynamics of thermoenzymes, including the thermoalkalophilic L1 lipase derived from Bacillus stearothermophilus. We will also discuss the potential of using pressure for the analysis of the stability of thermoenzymes because high pressure is also important for the processing and preservation of foods. |
| first_indexed | 2025-11-15T09:24:35Z |
| format | Article |
| id | upm-34582 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:24:35Z |
| publishDate | 2014 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-345822015-12-16T02:27:57Z http://psasir.upm.edu.my/id/eprint/34582/ Molecular dynamics of thermoenzymes at high temperature and pressure: a review Karjiban, Roghayeh Abedi Lim, Wui Zhuan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Lipases are known for their versatility in addition to their ability to digest fat. They can be used for the formulation of detergents, as food ingredients and as biocatalysts in many industrial processes. Because conventional enzymes are frangible at high temperatures, the replacement of conventional chemical routes with biochemical processes that utilize thermostable lipases is vital in the industrial setting. Recent theoretical studies on enzymes have provided numerous fundamental insights into the structures, folding mechanisms and stabilities of these proteins. The studies corroborate the experimental results and provide additional information regarding the structures that were determined experimentally. In this paper, we review the computational studies that have described how temperature affects the structure and dynamics of thermoenzymes, including the thermoalkalophilic L1 lipase derived from Bacillus stearothermophilus. We will also discuss the potential of using pressure for the analysis of the stability of thermoenzymes because high pressure is also important for the processing and preservation of foods. Springer 2014-08 Article PeerReviewed Karjiban, Roghayeh Abedi and Lim, Wui Zhuan and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin (2014) Molecular dynamics of thermoenzymes at high temperature and pressure: a review. The Protein Journal, 33 (4). pp. 369-376. ISSN 1572-3887; ESSN: 1573-4943 http://link.springer.com/article/10.1007%2Fs10930-014-9568-8 10.1007/s10930-014-9568-8 |
| spellingShingle | Karjiban, Roghayeh Abedi Lim, Wui Zhuan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title | Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title_full | Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title_fullStr | Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title_full_unstemmed | Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title_short | Molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| title_sort | molecular dynamics of thermoenzymes at high temperature and pressure: a review |
| url | http://psasir.upm.edu.my/id/eprint/34582/ http://psasir.upm.edu.my/id/eprint/34582/ http://psasir.upm.edu.my/id/eprint/34582/ |