MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction
Background: MicroRNAs (miRNAs) have a crucial role in gene expression regulation and protein synthesis, especially in the central nervous system. In developing mouse embryos a novel miRNA, miR-3099, is highly expressed, particularly in the central nervous system. This study aims to determine the exp...
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| Format: | Article |
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Penerbit Universiti Sains Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34528/ |
| _version_ | 1848847798165831680 |
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| author | Zainal Abidin, Shahidee Abbaspourbabaei, Maryam Ntimi, Carolindah Makena Siew, Wei Hong Cheah , Pike See Rosli, Rozita Nordin, Norshariza Ling, King Hwa |
| author_facet | Zainal Abidin, Shahidee Abbaspourbabaei, Maryam Ntimi, Carolindah Makena Siew, Wei Hong Cheah , Pike See Rosli, Rozita Nordin, Norshariza Ling, King Hwa |
| author_sort | Zainal Abidin, Shahidee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: MicroRNAs (miRNAs) have a crucial role in gene expression regulation and protein synthesis, especially in the central nervous system. In developing mouse embryos a novel miRNA, miR-3099, is highly expressed, particularly in the central nervous system. This study aims to determine the expression of miR-3099 during cellular differentiation of 46C mouse embryonic stem cells after neural induction with N2/B27 medium.
Methods: 46C mouse embryonic stem cells were subjected to neural induction with N2/B27 medium. At 0, 3, 7, 11, 17, and 22 days after neural induction, the cells were screened for various pluripotent, progenitor, and differentiating/differentiated cells markers by immunocytochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR). Stem-loop pulse RT-PCR was performed to determine the expression of miR-3099 at all selected time points after neural induction.
Results: Our findings showed that after induction, mouse embryonic stem cells differentiated into heterogeneous pools of cells containing neurons, astrocytes, and oligodendrocytes. Mouse embryonic stem cells and neural progenitor/precursor cells were also present in culture up to day 22 as indicated by RT-PCR analysis. Elucidation of miR-3099 expression during in vitro neural induction revealed that this miRNA was expressed throughout the differentiation process of 46C mouse embryonic stem cells. miR-3099 was expressed at higher levels on day 11, 17, and 22 as compared to day 0, 3 and 7 after neural induction.
Conclusion: The level of miR-3099 expression was higher in differentiated mouse embryonic stem cells after neural induction. This finding suggested that miR-3099 might play a role in regulating neural stem cell differentiation. However, further characterisation of miR-3099 in a better characterised or optimised differentiated neural stem cell culture would provide increased understanding of the cellular function and molecular targets of miR-3099, especially in neuron development. |
| first_indexed | 2025-11-15T09:24:19Z |
| format | Article |
| id | upm-34528 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:24:19Z |
| publishDate | 2014 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-345282015-12-15T08:50:57Z http://psasir.upm.edu.my/id/eprint/34528/ MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction Zainal Abidin, Shahidee Abbaspourbabaei, Maryam Ntimi, Carolindah Makena Siew, Wei Hong Cheah , Pike See Rosli, Rozita Nordin, Norshariza Ling, King Hwa Background: MicroRNAs (miRNAs) have a crucial role in gene expression regulation and protein synthesis, especially in the central nervous system. In developing mouse embryos a novel miRNA, miR-3099, is highly expressed, particularly in the central nervous system. This study aims to determine the expression of miR-3099 during cellular differentiation of 46C mouse embryonic stem cells after neural induction with N2/B27 medium. Methods: 46C mouse embryonic stem cells were subjected to neural induction with N2/B27 medium. At 0, 3, 7, 11, 17, and 22 days after neural induction, the cells were screened for various pluripotent, progenitor, and differentiating/differentiated cells markers by immunocytochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR). Stem-loop pulse RT-PCR was performed to determine the expression of miR-3099 at all selected time points after neural induction. Results: Our findings showed that after induction, mouse embryonic stem cells differentiated into heterogeneous pools of cells containing neurons, astrocytes, and oligodendrocytes. Mouse embryonic stem cells and neural progenitor/precursor cells were also present in culture up to day 22 as indicated by RT-PCR analysis. Elucidation of miR-3099 expression during in vitro neural induction revealed that this miRNA was expressed throughout the differentiation process of 46C mouse embryonic stem cells. miR-3099 was expressed at higher levels on day 11, 17, and 22 as compared to day 0, 3 and 7 after neural induction. Conclusion: The level of miR-3099 expression was higher in differentiated mouse embryonic stem cells after neural induction. This finding suggested that miR-3099 might play a role in regulating neural stem cell differentiation. However, further characterisation of miR-3099 in a better characterised or optimised differentiated neural stem cell culture would provide increased understanding of the cellular function and molecular targets of miR-3099, especially in neuron development. Penerbit Universiti Sains Malaysia 2014-12 Article PeerReviewed Zainal Abidin, Shahidee and Abbaspourbabaei, Maryam and Ntimi, Carolindah Makena and Siew, Wei Hong and Cheah , Pike See and Rosli, Rozita and Nordin, Norshariza and Ling, King Hwa (2014) MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction. Malaysian Journal of Medical Sciences, 21 (spec.). pp. 27-33. ISSN 1394-195X; ESSN: 2180-4303 http://www.mjms.usm.my/default.asp?tag=14&kod_volume=78 |
| spellingShingle | Zainal Abidin, Shahidee Abbaspourbabaei, Maryam Ntimi, Carolindah Makena Siew, Wei Hong Cheah , Pike See Rosli, Rozita Nordin, Norshariza Ling, King Hwa MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title | MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title_full | MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title_fullStr | MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title_full_unstemmed | MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title_short | MiR-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| title_sort | mir-3099 is overexpressed in differentiating 46c mouse embryonic stem cells upon neural induction |
| url | http://psasir.upm.edu.my/id/eprint/34528/ http://psasir.upm.edu.my/id/eprint/34528/ |