Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines
This work aims to establish microscale methods to rapidly explore bioprocess options that might be used to enhance bioconversion reaction yields: either by shifting unfavourable reaction equilibria or by overcoming substrate and/or product inhibition. As a typical and industrially relevant example o...
| Main Authors: | , , , , |
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| Format: | Article |
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Springer
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34517/ |
| _version_ | 1848847795184730112 |
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| author | Halim, Murni Rios-Solis, Leonardo Micheletti, Martina Ward, John Montgomery Lye, Gary J. |
| author_facet | Halim, Murni Rios-Solis, Leonardo Micheletti, Martina Ward, John Montgomery Lye, Gary J. |
| author_sort | Halim, Murni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This work aims to establish microscale methods to rapidly explore bioprocess options that might be used to enhance bioconversion reaction yields: either by shifting unfavourable reaction equilibria or by overcoming substrate and/or product inhibition. As a typical and industrially relevant example of the problems faced we have examined the asymmetric synthesis of (2S,3R)-2-amino-1,3,4-butanetriol from l-erythrulose using the ω-transaminase from Chromobacterium violaceum DSM30191 (CV2025 ω-TAm) and methylbenzylamine as the amino donor. The first process option involves the use of alternative amino donors. The second couples the CV2025 ω-TAm with alcohol dehydrogenase and glucose dehydrogenase for removal of the acetophenone (AP) by-product by in situ conversion to (R)-1-phenylethanol. The final approaches involve physical in-situ product removal methods. Reduced pressure conditions, attained using a 96-well vacuum manifold were used to selectively increase evaporation of the volatile AP while polymeric resins were also utilised for selective adsorption of AP from the bioconversion medium. For the particular reaction studied here the most promising bioprocess options were use of an alternative amino donor, such as isopropylamine, which enabled a 2.8-fold increase in reaction yield, or use of a second enzyme system which achieved a 3.3-fold increase in yield. |
| first_indexed | 2025-11-15T09:24:17Z |
| format | Article |
| id | upm-34517 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:24:17Z |
| publishDate | 2014 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-345172015-12-15T07:58:38Z http://psasir.upm.edu.my/id/eprint/34517/ Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines Halim, Murni Rios-Solis, Leonardo Micheletti, Martina Ward, John Montgomery Lye, Gary J. This work aims to establish microscale methods to rapidly explore bioprocess options that might be used to enhance bioconversion reaction yields: either by shifting unfavourable reaction equilibria or by overcoming substrate and/or product inhibition. As a typical and industrially relevant example of the problems faced we have examined the asymmetric synthesis of (2S,3R)-2-amino-1,3,4-butanetriol from l-erythrulose using the ω-transaminase from Chromobacterium violaceum DSM30191 (CV2025 ω-TAm) and methylbenzylamine as the amino donor. The first process option involves the use of alternative amino donors. The second couples the CV2025 ω-TAm with alcohol dehydrogenase and glucose dehydrogenase for removal of the acetophenone (AP) by-product by in situ conversion to (R)-1-phenylethanol. The final approaches involve physical in-situ product removal methods. Reduced pressure conditions, attained using a 96-well vacuum manifold were used to selectively increase evaporation of the volatile AP while polymeric resins were also utilised for selective adsorption of AP from the bioconversion medium. For the particular reaction studied here the most promising bioprocess options were use of an alternative amino donor, such as isopropylamine, which enabled a 2.8-fold increase in reaction yield, or use of a second enzyme system which achieved a 3.3-fold increase in yield. Springer 2014-05 Article PeerReviewed Halim, Murni and Rios-Solis, Leonardo and Micheletti, Martina and Ward, John Montgomery and Lye, Gary J. (2014) Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines. Bioprocess and Biosystems Engineering, 37 (5). pp. 931-941. ISSN 1615-7591; ESSN: 1615-7605 http://link.springer.com/article/10.1007%2Fs00449-013-1065-5 10.1007/s00449-013-1065-5 |
| spellingShingle | Halim, Murni Rios-Solis, Leonardo Micheletti, Martina Ward, John Montgomery Lye, Gary J. Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title | Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title_full | Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title_fullStr | Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title_full_unstemmed | Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title_short | Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| title_sort | microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines |
| url | http://psasir.upm.edu.my/id/eprint/34517/ http://psasir.upm.edu.my/id/eprint/34517/ http://psasir.upm.edu.my/id/eprint/34517/ |