Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants
Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in b...
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Japanese Society of Breeding
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34510/ http://psasir.upm.edu.my/id/eprint/34510/1/Overexpression%20of%20Arabidopsis%20thaliana%20gibberellic%20acid%2020%20oxidase%20%28AtGA20ox%29%20gene%20enhance%20the%20vegetative%20growth%20and%20fiber%20quality%20in%20kenaf%20%28Hibiscus%20cannabinus%20L.%29%20plants.pdf |
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| author | Withanage, Samanthi Priyanka Hossain, Md. Aktar Muniandi, Sures Kumar Roslan, Hairul Azman Abdullah, Mohammad Puad Napis, Suhaimi Ab Shukor, Nor Aini |
| author_facet | Withanage, Samanthi Priyanka Hossain, Md. Aktar Muniandi, Sures Kumar Roslan, Hairul Azman Abdullah, Mohammad Puad Napis, Suhaimi Ab Shukor, Nor Aini |
| author_sort | Withanage, Samanthi Priyanka |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3ā1.52 ng gā1 fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants. |
| first_indexed | 2025-11-15T09:24:15Z |
| format | Article |
| id | upm-34510 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:24:15Z |
| publishDate | 2015 |
| publisher | Japanese Society of Breeding |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-345102016-09-19T09:46:10Z http://psasir.upm.edu.my/id/eprint/34510/ Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants Withanage, Samanthi Priyanka Hossain, Md. Aktar Muniandi, Sures Kumar Roslan, Hairul Azman Abdullah, Mohammad Puad Napis, Suhaimi Ab Shukor, Nor Aini Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3ā1.52 ng gā1 fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants. Japanese Society of Breeding 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34510/1/Overexpression%20of%20Arabidopsis%20thaliana%20gibberellic%20acid%2020%20oxidase%20%28AtGA20ox%29%20gene%20enhance%20the%20vegetative%20growth%20and%20fiber%20quality%20in%20kenaf%20%28Hibiscus%20cannabinus%20L.%29%20plants.pdf Withanage, Samanthi Priyanka and Hossain, Md. Aktar and Muniandi, Sures Kumar and Roslan, Hairul Azman and Abdullah, Mohammad Puad and Napis, Suhaimi and Ab Shukor, Nor Aini (2015) Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants. Breeding Science, 65 (3). pp. 177-191. ISSN 1344-7610; ESSN: 1347-3735 https://www.jstage.jst.go.jp/article/jsbbs/65/3/65_177/_article 10.1270/jsbbs.65.177 |
| spellingShingle | Withanage, Samanthi Priyanka Hossain, Md. Aktar Muniandi, Sures Kumar Roslan, Hairul Azman Abdullah, Mohammad Puad Napis, Suhaimi Ab Shukor, Nor Aini Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title | Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title_full | Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title_fullStr | Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title_full_unstemmed | Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title_short | Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants |
| title_sort | overexpression of arabidopsis thaliana gibberellic acid 20 oxidase (atga20ox) gene enhance the vegetative growth and fiber quality in kenaf (hibiscus cannabinus l.) plants |
| url | http://psasir.upm.edu.my/id/eprint/34510/ http://psasir.upm.edu.my/id/eprint/34510/ http://psasir.upm.edu.my/id/eprint/34510/ http://psasir.upm.edu.my/id/eprint/34510/1/Overexpression%20of%20Arabidopsis%20thaliana%20gibberellic%20acid%2020%20oxidase%20%28AtGA20ox%29%20gene%20enhance%20the%20vegetative%20growth%20and%20fiber%20quality%20in%20kenaf%20%28Hibiscus%20cannabinus%20L.%29%20plants.pdf |