Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the...
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| Format: | Article |
| Language: | English |
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Scientific and Technical Research Council of Turkey
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34443/ http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf |
| _version_ | 1848847774167072768 |
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| author | Shabanimofrad, Mahmoodreza Yusop, Mohd Rafii Ashkani, Sadegh Musa, Mohamed Hanafi Adam, Nur Azura Iffah Haifa, Harun, Abdul Rahim Abdul Latif, Mohammad |
| author_facet | Shabanimofrad, Mahmoodreza Yusop, Mohd Rafii Ashkani, Sadegh Musa, Mohamed Hanafi Adam, Nur Azura Iffah Haifa, Harun, Abdul Rahim Abdul Latif, Mohammad |
| author_sort | Shabanimofrad, Mahmoodreza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars. |
| first_indexed | 2025-11-15T09:23:57Z |
| format | Article |
| id | upm-34443 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:23:57Z |
| publishDate | 2014 |
| publisher | Scientific and Technical Research Council of Turkey |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-344432016-01-27T02:12:24Z http://psasir.upm.edu.my/id/eprint/34443/ Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers Shabanimofrad, Mahmoodreza Yusop, Mohd Rafii Ashkani, Sadegh Musa, Mohamed Hanafi Adam, Nur Azura Iffah Haifa, Harun, Abdul Rahim Abdul Latif, Mohammad Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars. Scientific and Technical Research Council of Turkey 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf Shabanimofrad, Mahmoodreza and Yusop, Mohd Rafii and Ashkani, Sadegh and Musa, Mohamed Hanafi and Adam, Nur Azura and Iffah Haifa, and Harun, Abdul Rahim and Abdul Latif, Mohammad (2014) Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers. Turkish Journal of Biology, 39 (5). pp. 666-673. ISSN 1300-0152; ESSN: 1303-6092 http://journals.tubitak.gov.tr/biology/abstract.htm?id=16911 10.3906/biy-1406-78 |
| spellingShingle | Shabanimofrad, Mahmoodreza Yusop, Mohd Rafii Ashkani, Sadegh Musa, Mohamed Hanafi Adam, Nur Azura Iffah Haifa, Harun, Abdul Rahim Abdul Latif, Mohammad Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title | Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title_full | Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title_fullStr | Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title_full_unstemmed | Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title_short | Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers |
| title_sort | marker-assisted selection for rice brown planthopper (nilaparvata lugens)resistance using linked ssr markers |
| url | http://psasir.upm.edu.my/id/eprint/34443/ http://psasir.upm.edu.my/id/eprint/34443/ http://psasir.upm.edu.my/id/eprint/34443/ http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf |