MALDI-target integrated platform for affinity-captured protein digestion
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, diges...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Published: |
Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34429/ |
| _version_ | 1848847770102792192 |
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| author | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas |
| author_facet | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas |
| author_sort | Ahmad Tajudin, Asilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. |
| first_indexed | 2025-11-15T09:23:53Z |
| format | Article |
| id | upm-34429 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:23:53Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-344292015-12-15T03:02:55Z http://psasir.upm.edu.my/id/eprint/34429/ MALDI-target integrated platform for affinity-captured protein digestion Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. Elsevier 2014-01-07 Article PeerReviewed Ahmad Tajudin, Asilah and Adler, Belinda and Ekstrom, Simon and Marko-Varga, Gyorgy and Malm, Johan and Lilja, Hans and Laurell, Thomas (2014) MALDI-target integrated platform for affinity-captured protein digestion. Analytica Chimica Acta, 807. pp. 1-8. ISSN 0003-2670; ESSN: 1873-4324 http://www.sciencedirect.com/science/article/pii/S0003267013011689 10.1016/j.aca.2013.08.051 |
| spellingShingle | Ahmad Tajudin, Asilah Adler, Belinda Ekstrom, Simon Marko-Varga, Gyorgy Malm, Johan Lilja, Hans Laurell, Thomas MALDI-target integrated platform for affinity-captured protein digestion |
| title | MALDI-target integrated platform for affinity-captured protein digestion |
| title_full | MALDI-target integrated platform for affinity-captured protein digestion |
| title_fullStr | MALDI-target integrated platform for affinity-captured protein digestion |
| title_full_unstemmed | MALDI-target integrated platform for affinity-captured protein digestion |
| title_short | MALDI-target integrated platform for affinity-captured protein digestion |
| title_sort | maldi-target integrated platform for affinity-captured protein digestion |
| url | http://psasir.upm.edu.my/id/eprint/34429/ http://psasir.upm.edu.my/id/eprint/34429/ http://psasir.upm.edu.my/id/eprint/34429/ |