MALDI-target integrated platform for affinity-captured protein digestion

MALDI-target integrated platform for affinity-captured protein digestion

To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, diges...

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Bibliographic Details
Main Authors: Ahmad Tajudin, Asilah, Adler, Belinda, Ekstrom, Simon, Marko-Varga, Gyorgy, Malm, Johan, Lilja, Hans, Laurell, Thomas
Format: Article
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34429/
Description
Summary:To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.

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