Linearity study on detection and quantification limits for the determination of avermectins using linear regression
In this study, linear relationships between response and concentration were used to estimate the detection limit (DL) and quantification limit (QL) for five avermectins: emamectin, abamectin, doramectin, moxidectin, and ivermectin. Estimation of DL and QL was based on the standard deviation of resid...
| Main Authors: | , , , |
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| Format: | Article |
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National Laboratories of Foods and Drugs
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34385/ |
| _version_ | 1848847757747421184 |
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| author | Ismail, Rafidah Lee, Hai Yen Mahyudin, Nor Ainy Abu Bakar, Fatimah |
| author_facet | Ismail, Rafidah Lee, Hai Yen Mahyudin, Nor Ainy Abu Bakar, Fatimah |
| author_sort | Ismail, Rafidah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, linear relationships between response and concentration were used to estimate the detection limit (DL) and quantification limit (QL) for five avermectins: emamectin, abamectin, doramectin, moxidectin, and ivermectin. Estimation of DL and QL was based on the standard deviation of residual and y-intercept of the regression line at low concentrations of avermectins, using the dispersive solid-phase extraction procedure. Avermectin extracts were analyzed using liquid chromatography tandem mass spectrometry. Based on the regression slope, DL and QL were higher at concentrations of 0.3–0.4 μg/kg and 1 μg/kg, respectively, for all avermectin compounds. Linearity assessment was performed by linear regression, which incorporated a regression model, outlier rejection, and evaluation of the assumption with a significant test. For all avermectins, there is a significant correlation between response and concentration in the range 1–15 μg/kg, and the y-intercept passes through origin (zero). |
| first_indexed | 2025-11-15T09:23:41Z |
| format | Article |
| id | upm-34385 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:23:41Z |
| publishDate | 2014 |
| publisher | National Laboratories of Foods and Drugs |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-343852015-12-15T01:32:22Z http://psasir.upm.edu.my/id/eprint/34385/ Linearity study on detection and quantification limits for the determination of avermectins using linear regression Ismail, Rafidah Lee, Hai Yen Mahyudin, Nor Ainy Abu Bakar, Fatimah In this study, linear relationships between response and concentration were used to estimate the detection limit (DL) and quantification limit (QL) for five avermectins: emamectin, abamectin, doramectin, moxidectin, and ivermectin. Estimation of DL and QL was based on the standard deviation of residual and y-intercept of the regression line at low concentrations of avermectins, using the dispersive solid-phase extraction procedure. Avermectin extracts were analyzed using liquid chromatography tandem mass spectrometry. Based on the regression slope, DL and QL were higher at concentrations of 0.3–0.4 μg/kg and 1 μg/kg, respectively, for all avermectin compounds. Linearity assessment was performed by linear regression, which incorporated a regression model, outlier rejection, and evaluation of the assumption with a significant test. For all avermectins, there is a significant correlation between response and concentration in the range 1–15 μg/kg, and the y-intercept passes through origin (zero). National Laboratories of Foods and Drugs 2014-12 Article PeerReviewed Ismail, Rafidah and Lee, Hai Yen and Mahyudin, Nor Ainy and Abu Bakar, Fatimah (2014) Linearity study on detection and quantification limits for the determination of avermectins using linear regression. Journal of Food and Drug Analysis, 22 (4). pp. 407-412. ISSN 1021-9498 http://www.sciencedirect.com/science/article/pii/S1021949814000519 10.1016/j.jfda.2014.01.026 |
| spellingShingle | Ismail, Rafidah Lee, Hai Yen Mahyudin, Nor Ainy Abu Bakar, Fatimah Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title | Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title_full | Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title_fullStr | Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title_full_unstemmed | Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title_short | Linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| title_sort | linearity study on detection and quantification limits for the determination of avermectins using linear regression |
| url | http://psasir.upm.edu.my/id/eprint/34385/ http://psasir.upm.edu.my/id/eprint/34385/ http://psasir.upm.edu.my/id/eprint/34385/ |