Intracellular production of IFN-alpha 2b in Lactococcus lactis
Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using g...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Netherlands
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/34246/ |
| _version_ | 1848847717209473024 |
|---|---|
| author | Bayat, Omid Baradaran, Ali Ariff, Arbakariya Mohamad, Rosfarizan Abdul Rahim, Raha |
| author_facet | Bayat, Omid Baradaran, Ali Ariff, Arbakariya Mohamad, Rosfarizan Abdul Rahim, Raha |
| author_sort | Bayat, Omid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using glucose for growth of both recombinant strains with nisin, used for induction of the recombinant strain with Pnis promoter, at 30 ng/ml. The optimum time for MG:IFN was 9 h and for NZ:IFN was 4.5 h. The highest productions by MG:IFN and NZ:IFN were 1.9 and 2.4 μg IFN/l, respectively. Both of the expressed IFNs showed bioactivities of 1.9 × 10(6) IU/mg that were acceptable for further clinical studies. |
| first_indexed | 2025-11-15T09:23:02Z |
| format | Article |
| id | upm-34246 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:23:02Z |
| publishDate | 2014 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-342462015-12-10T00:46:25Z http://psasir.upm.edu.my/id/eprint/34246/ Intracellular production of IFN-alpha 2b in Lactococcus lactis Bayat, Omid Baradaran, Ali Ariff, Arbakariya Mohamad, Rosfarizan Abdul Rahim, Raha Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using glucose for growth of both recombinant strains with nisin, used for induction of the recombinant strain with Pnis promoter, at 30 ng/ml. The optimum time for MG:IFN was 9 h and for NZ:IFN was 4.5 h. The highest productions by MG:IFN and NZ:IFN were 1.9 and 2.4 μg IFN/l, respectively. Both of the expressed IFNs showed bioactivities of 1.9 × 10(6) IU/mg that were acceptable for further clinical studies. Springer Netherlands 2014-03 Article PeerReviewed Bayat, Omid and Baradaran, Ali and Ariff, Arbakariya and Mohamad, Rosfarizan and Abdul Rahim, Raha (2014) Intracellular production of IFN-alpha 2b in Lactococcus lactis. Biotechnology Letters, 36 (3). pp. 581-585. ISSN 0141-5492; ESSN: 1573-6776 http://link.springer.com/article/10.1007/s10529-013-1390-4 10.1007/s10529-013-1390-4 |
| spellingShingle | Bayat, Omid Baradaran, Ali Ariff, Arbakariya Mohamad, Rosfarizan Abdul Rahim, Raha Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title | Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title_full | Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title_fullStr | Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title_full_unstemmed | Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title_short | Intracellular production of IFN-alpha 2b in Lactococcus lactis |
| title_sort | intracellular production of ifn-alpha 2b in lactococcus lactis |
| url | http://psasir.upm.edu.my/id/eprint/34246/ http://psasir.upm.edu.my/id/eprint/34246/ http://psasir.upm.edu.my/id/eprint/34246/ |