Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross”
In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata “KY Cros...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Japanese Society for Plant Cell and Molecular Biology
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34197/ http://psasir.upm.edu.my/id/eprint/34197/1/Influence%20of%20phenyl-urea%20and%20adenine-type%20cytokinins%20on%20direct%20adventitious%20shoot%20regeneration%20of%20cabbage%20%28Brassica%20oleracea%20subsp.%20capitata%29%20%E2%80%9CKY%20Cross%E2%80%9D.pdf |
| _version_ | 1848847702667821056 |
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| author | Ravanfar, Seyed Ali Salim, Shahida Abdul Aziz, Maheran Abdullah, Siti Nor Akmar Abdul Rashid, Azmi |
| author_facet | Ravanfar, Seyed Ali Salim, Shahida Abdul Aziz, Maheran Abdullah, Siti Nor Akmar Abdul Rashid, Azmi |
| author_sort | Ravanfar, Seyed Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 μM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 μM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”. |
| first_indexed | 2025-11-15T09:22:48Z |
| format | Article |
| id | upm-34197 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:22:48Z |
| publishDate | 2014 |
| publisher | Japanese Society for Plant Cell and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-341972016-09-15T03:05:17Z http://psasir.upm.edu.my/id/eprint/34197/ Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” Ravanfar, Seyed Ali Salim, Shahida Abdul Aziz, Maheran Abdullah, Siti Nor Akmar Abdul Rashid, Azmi In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 μM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 μM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”. Japanese Society for Plant Cell and Molecular Biology 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34197/1/Influence%20of%20phenyl-urea%20and%20adenine-type%20cytokinins%20on%20direct%20adventitious%20shoot%20regeneration%20of%20cabbage%20%28Brassica%20oleracea%20subsp.%20capitata%29%20%E2%80%9CKY%20Cross%E2%80%9D.pdf Ravanfar, Seyed Ali and Salim, Shahida and Abdul Aziz, Maheran and Abdullah, Siti Nor Akmar and Abdul Rashid, Azmi (2014) Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross”. Plant Biotechnology, 31 (3). pp. 275-280. ISSN 1342-4580; ESSN: 1347-6114 https://www.jstage.jst.go.jp/article/plantbiotechnology/31/3/31_14.0514a/_article 10.5511/plantbiotechnology.14.0514a |
| spellingShingle | Ravanfar, Seyed Ali Salim, Shahida Abdul Aziz, Maheran Abdullah, Siti Nor Akmar Abdul Rashid, Azmi Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title | Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title_full | Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title_fullStr | Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title_full_unstemmed | Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title_short | Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross” |
| title_sort | influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (brassica oleracea subsp. capitata) “ky cross” |
| url | http://psasir.upm.edu.my/id/eprint/34197/ http://psasir.upm.edu.my/id/eprint/34197/ http://psasir.upm.edu.my/id/eprint/34197/ http://psasir.upm.edu.my/id/eprint/34197/1/Influence%20of%20phenyl-urea%20and%20adenine-type%20cytokinins%20on%20direct%20adventitious%20shoot%20regeneration%20of%20cabbage%20%28Brassica%20oleracea%20subsp.%20capitata%29%20%E2%80%9CKY%20Cross%E2%80%9D.pdf |