In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract

We investigate the biostability of phenolic acids from a kenaf (Hibiscus cannabinus L.) seed extract using an in vitro model simulating the physicochemical (pH, temperature and bile salts) and biological (gastric and pancreatic enzymes) gastrointestinal conditions. Some of the antioxidants in the ke...

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Main Authors: Wong, Yu Hua, Tan, Chin Ping, Long, Kamariah, Nyam, Kar Lin
Format: Article
Published: Czech Academy of Agricultural Sciences 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34150/
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author Wong, Yu Hua
Tan, Chin Ping
Long, Kamariah
Nyam, Kar Lin
author_facet Wong, Yu Hua
Tan, Chin Ping
Long, Kamariah
Nyam, Kar Lin
author_sort Wong, Yu Hua
building UPM Institutional Repository
collection Online Access
description We investigate the biostability of phenolic acids from a kenaf (Hibiscus cannabinus L.) seed extract using an in vitro model simulating the physicochemical (pH, temperature and bile salts) and biological (gastric and pancreatic enzymes) gastrointestinal conditions. Some of the antioxidants in the kenaf seed extract were not relatively stable in the intestinal phase of the gastrointestinal tracts. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and total phenolic content (TPC) assays displayed similar trends as the biostability of phenolic acids, which decreased during the digestion process. The overall percentage loss of selected phenolic acids was 8.4–49.4% in the intestinal phase. For the overall in vitro digestion system, significant correlations between phenolic acids, total phenolic content (TPC) and antioxidant activity (DPPH) were obtained in all digestion phases with the exception of the gastric phase.
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institution Universiti Putra Malaysia
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publisher Czech Academy of Agricultural Sciences
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spelling upm-341502015-12-08T02:06:39Z http://psasir.upm.edu.my/id/eprint/34150/ In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract Wong, Yu Hua Tan, Chin Ping Long, Kamariah Nyam, Kar Lin We investigate the biostability of phenolic acids from a kenaf (Hibiscus cannabinus L.) seed extract using an in vitro model simulating the physicochemical (pH, temperature and bile salts) and biological (gastric and pancreatic enzymes) gastrointestinal conditions. Some of the antioxidants in the kenaf seed extract were not relatively stable in the intestinal phase of the gastrointestinal tracts. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and total phenolic content (TPC) assays displayed similar trends as the biostability of phenolic acids, which decreased during the digestion process. The overall percentage loss of selected phenolic acids was 8.4–49.4% in the intestinal phase. For the overall in vitro digestion system, significant correlations between phenolic acids, total phenolic content (TPC) and antioxidant activity (DPPH) were obtained in all digestion phases with the exception of the gastric phase. Czech Academy of Agricultural Sciences 2014 Article PeerReviewed Wong, Yu Hua and Tan, Chin Ping and Long, Kamariah and Nyam, Kar Lin (2014) In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract. Czech Journal of Food Sciences, 32 (2). pp. 177-181. ISSN 1212-1800; ESSN: 1805-9317 http://www.agriculturejournals.cz/web/cjfs.htm?volume=32&firstPage=177&type=publishedArticle
spellingShingle Wong, Yu Hua
Tan, Chin Ping
Long, Kamariah
Nyam, Kar Lin
In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title_full In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title_fullStr In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title_full_unstemmed In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title_short In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract
title_sort in vitro simulated digestion on the biostability of hibiscus cannabinus l. seed extract
url http://psasir.upm.edu.my/id/eprint/34150/
http://psasir.upm.edu.my/id/eprint/34150/