Binding of copper (II) ion to cytochrome c
Binding of aquacopper(11) ion to horse and tuna ferricytochrome c has been investigated over the pH range 2-9. Between pH 4 and 7 the principal binding site for horse cytochrome has an intrinsic association constant of 1.8 × 104 1. mol-1 and pKa of 6.5±0.6 at 25ºC and ionic strength of 0.1. Binding...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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CSIRO Publishing
1981
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34013/ http://psasir.upm.edu.my/id/eprint/34013/1/34013%20binding%20of%20copper.pdf |
| _version_ | 1848847656255750144 |
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| author | Augustin, Mary Ann Yandell, John K. |
| author_facet | Augustin, Mary Ann Yandell, John K. |
| author_sort | Augustin, Mary Ann |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Binding of aquacopper(11) ion to horse and tuna ferricytochrome c has been investigated over the pH range 2-9. Between pH 4 and 7 the principal binding site for horse cytochrome has an intrinsic association constant of 1.8 × 104 1. mol-1 and pKa of 6.5±0.6 at 25ºC and ionic strength of 0.1. Binding to histidine 33 is consistent with this information, as well as with e.s.r., n.m.r. and visible absorption spectral data. Other sites also bind copper but with association constants at least a factor of ten smaller. At high pH (> 8) further binding sites predominate. |
| first_indexed | 2025-11-15T09:22:04Z |
| format | Article |
| id | upm-34013 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:22:04Z |
| publishDate | 1981 |
| publisher | CSIRO Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-340132015-04-15T04:29:35Z http://psasir.upm.edu.my/id/eprint/34013/ Binding of copper (II) ion to cytochrome c Augustin, Mary Ann Yandell, John K. Binding of aquacopper(11) ion to horse and tuna ferricytochrome c has been investigated over the pH range 2-9. Between pH 4 and 7 the principal binding site for horse cytochrome has an intrinsic association constant of 1.8 × 104 1. mol-1 and pKa of 6.5±0.6 at 25ºC and ionic strength of 0.1. Binding to histidine 33 is consistent with this information, as well as with e.s.r., n.m.r. and visible absorption spectral data. Other sites also bind copper but with association constants at least a factor of ten smaller. At high pH (> 8) further binding sites predominate. CSIRO Publishing 1981 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34013/1/34013%20binding%20of%20copper.pdf Augustin, Mary Ann and Yandell, John K. (1981) Binding of copper (II) ion to cytochrome c. Australian Journal of Chemistry, 34 (1). pp. 91-97. ISSN 0004-9425 |
| spellingShingle | Augustin, Mary Ann Yandell, John K. Binding of copper (II) ion to cytochrome c |
| title | Binding of copper (II) ion to cytochrome c |
| title_full | Binding of copper (II) ion to cytochrome c |
| title_fullStr | Binding of copper (II) ion to cytochrome c |
| title_full_unstemmed | Binding of copper (II) ion to cytochrome c |
| title_short | Binding of copper (II) ion to cytochrome c |
| title_sort | binding of copper (ii) ion to cytochrome c |
| url | http://psasir.upm.edu.my/id/eprint/34013/ http://psasir.upm.edu.my/id/eprint/34013/1/34013%20binding%20of%20copper.pdf |