Some kinetic studies on immobilized uricase
1. Uricase was immobilized onto glutaraldehyde-activated nylon tube. 2. The activity of the immobilized uricase tube was assayed by a plug-flow method adopting the integrated form of Michaelis-Menten equation. 3. Up to about 60% activity of the soluble enzyme is retained when the enzyme is derivat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
1981
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33896/ http://psasir.upm.edu.my/id/eprint/33896/1/33896%20some%20kinetic%20studies.pdf http://psasir.upm.edu.my/id/eprint/33896/7/33896.pdf |
| _version_ | 1848847625492627456 |
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| author | Salleh, Abu Bakar Ledingham, W. M. |
| author_facet | Salleh, Abu Bakar Ledingham, W. M. |
| author_sort | Salleh, Abu Bakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 1. Uricase was immobilized onto glutaraldehyde-activated nylon tube.
2. The activity of the immobilized uricase tube was assayed by a plug-flow method adopting the integrated form of Michaelis-Menten equation.
3. Up to about 60% activity of the soluble enzyme is retained when the enzyme is derivatized.
4. Effects of changing certain parameters in the assay system were also shown.
5. The immobilized uricase has an optium pH of about 9.0, and is quite stable at 37°C when stored in borate buffer pH 9.0 in the presence of NaCl and Triton × 100. |
| first_indexed | 2025-11-15T09:21:35Z |
| format | Article |
| id | upm-33896 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:21:35Z |
| publishDate | 1981 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-338962024-10-15T06:50:11Z http://psasir.upm.edu.my/id/eprint/33896/ Some kinetic studies on immobilized uricase Salleh, Abu Bakar Ledingham, W. M. 1. Uricase was immobilized onto glutaraldehyde-activated nylon tube. 2. The activity of the immobilized uricase tube was assayed by a plug-flow method adopting the integrated form of Michaelis-Menten equation. 3. Up to about 60% activity of the soluble enzyme is retained when the enzyme is derivatized. 4. Effects of changing certain parameters in the assay system were also shown. 5. The immobilized uricase has an optium pH of about 9.0, and is quite stable at 37°C when stored in borate buffer pH 9.0 in the presence of NaCl and Triton × 100. Elsevier 1981 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/33896/1/33896%20some%20kinetic%20studies.pdf text en http://psasir.upm.edu.my/id/eprint/33896/7/33896.pdf Salleh, Abu Bakar and Ledingham, W. M. (1981) Some kinetic studies on immobilized uricase. International Journal of Biochemistry, 13 (10). pp. 1113-1118. ISSN 0020-711X https://www.sciencedirect.com/science/article/pii/0020711X81901750?via%3Dihub 10.1016/0020-711X(81)90175-0 |
| spellingShingle | Salleh, Abu Bakar Ledingham, W. M. Some kinetic studies on immobilized uricase |
| title | Some kinetic studies on immobilized uricase |
| title_full | Some kinetic studies on immobilized uricase |
| title_fullStr | Some kinetic studies on immobilized uricase |
| title_full_unstemmed | Some kinetic studies on immobilized uricase |
| title_short | Some kinetic studies on immobilized uricase |
| title_sort | some kinetic studies on immobilized uricase |
| url | http://psasir.upm.edu.my/id/eprint/33896/ http://psasir.upm.edu.my/id/eprint/33896/ http://psasir.upm.edu.my/id/eprint/33896/ http://psasir.upm.edu.my/id/eprint/33896/1/33896%20some%20kinetic%20studies.pdf http://psasir.upm.edu.my/id/eprint/33896/7/33896.pdf |