Haem disorder in modified myoglobins
Apomyoglobin was reconstituted with deuterohaem derivatives under various con-ditions. The fraction of disordered component, which is characterized by a 1800 rotation of the haem group, for the various preparations was determined by n.m.r. spectroscopy. By using the procedures described, it was show...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Portland Press
1983
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33784/ http://psasir.upm.edu.my/id/eprint/33784/1/haem%20disorder.pdf |
| _version_ | 1848847595052466176 |
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| author | Ahmad, Mansor Kincaid, James R. |
| author_facet | Ahmad, Mansor Kincaid, James R. |
| author_sort | Ahmad, Mansor |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Apomyoglobin was reconstituted with deuterohaem derivatives under various con-ditions. The fraction of disordered component, which is characterized by a 1800 rotation of the haem group, for the various preparations was determined by n.m.r. spectroscopy. By using the procedures described, it was shown that the fraction of disordered component is minimized if the reconstitution is carried out with high-spin ferric haem derivatives within an experimentally determined optimum pH range of 8-9.5. Use of low-spin derivatives in either the ferrous or erric forms leads to substantial increases in the fraction of disordered form. Attempted removal of the disordered form by selective oxidation and chromatographic purification was not effective. |
| first_indexed | 2025-11-15T09:21:06Z |
| format | Article |
| id | upm-33784 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:21:06Z |
| publishDate | 1983 |
| publisher | Portland Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-337842015-04-16T07:19:08Z http://psasir.upm.edu.my/id/eprint/33784/ Haem disorder in modified myoglobins Ahmad, Mansor Kincaid, James R. Apomyoglobin was reconstituted with deuterohaem derivatives under various con-ditions. The fraction of disordered component, which is characterized by a 1800 rotation of the haem group, for the various preparations was determined by n.m.r. spectroscopy. By using the procedures described, it was shown that the fraction of disordered component is minimized if the reconstitution is carried out with high-spin ferric haem derivatives within an experimentally determined optimum pH range of 8-9.5. Use of low-spin derivatives in either the ferrous or erric forms leads to substantial increases in the fraction of disordered form. Attempted removal of the disordered form by selective oxidation and chromatographic purification was not effective. Portland Press 1983-04-22 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33784/1/haem%20disorder.pdf Ahmad, Mansor and Kincaid, James R. (1983) Haem disorder in modified myoglobins. Biochemical Journal, 215 (1). pp. 117-122. ISSN 0264-6021 http://www.biochemj.org/bj/215/bj2150117.htm |
| spellingShingle | Ahmad, Mansor Kincaid, James R. Haem disorder in modified myoglobins |
| title | Haem disorder in modified myoglobins |
| title_full | Haem disorder in modified myoglobins |
| title_fullStr | Haem disorder in modified myoglobins |
| title_full_unstemmed | Haem disorder in modified myoglobins |
| title_short | Haem disorder in modified myoglobins |
| title_sort | haem disorder in modified myoglobins |
| url | http://psasir.upm.edu.my/id/eprint/33784/ http://psasir.upm.edu.my/id/eprint/33784/ http://psasir.upm.edu.my/id/eprint/33784/1/haem%20disorder.pdf |