Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations
The present study was conducted to assess the capacitation status of frozen-thawed Bali spermatozoa in different heparin concentration with a short incubation time. Frozen thawed spermatozoa from Bali bulls were treated with either 25, 50 or 100 ug/ml heparin for 30 min. Capacitation was evaluated b...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33605/ http://psasir.upm.edu.my/id/eprint/33605/1/33605.pdf |
| _version_ | 1848847543693213696 |
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| author | Sarsaifi, Kazhal Haron, Abd Wahid Yusoff, Rosnina Hani, Homayoun Yimer, Nurhusien Naing, Soh Win Vejayan, Jaya Othman, Abas Mazni |
| author_facet | Sarsaifi, Kazhal Haron, Abd Wahid Yusoff, Rosnina Hani, Homayoun Yimer, Nurhusien Naing, Soh Win Vejayan, Jaya Othman, Abas Mazni |
| author_sort | Sarsaifi, Kazhal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study was conducted to assess the capacitation status of frozen-thawed Bali spermatozoa in different heparin concentration with a short incubation time. Frozen thawed spermatozoa from Bali bulls were treated with either 25, 50 or 100 ug/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and heterologous in vitro fertilization test] fertilization percentage (FP) and fertilization index (FI)]. Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with naphthol yellow S- erythrosine B to evaluate the response as compared with control (without heparin). Significantly higher percentage of acrosome reaction (AR) spermatozoa was observed after heparin treatment (43.8 - 60.2%) as compared with control (11.6%; p < 0.01). Also, a
significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 ug/ml heparin (68.4 and 73.6% fertilization percentage, respectively) as compared with control and 25 ug/ml heparin (13.5 and 41% cleavage rate; p < 0.01). It is concluded that Bali spermatozoa capacitated with 50-100 ug/ml heparin had significantly higher ability to improve capacitation status and acrosome reaction. |
| first_indexed | 2025-11-15T09:20:17Z |
| format | Conference or Workshop Item |
| id | upm-33605 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:20:17Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-336052020-07-22T02:28:46Z http://psasir.upm.edu.my/id/eprint/33605/ Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations Sarsaifi, Kazhal Haron, Abd Wahid Yusoff, Rosnina Hani, Homayoun Yimer, Nurhusien Naing, Soh Win Vejayan, Jaya Othman, Abas Mazni The present study was conducted to assess the capacitation status of frozen-thawed Bali spermatozoa in different heparin concentration with a short incubation time. Frozen thawed spermatozoa from Bali bulls were treated with either 25, 50 or 100 ug/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and heterologous in vitro fertilization test] fertilization percentage (FP) and fertilization index (FI)]. Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with naphthol yellow S- erythrosine B to evaluate the response as compared with control (without heparin). Significantly higher percentage of acrosome reaction (AR) spermatozoa was observed after heparin treatment (43.8 - 60.2%) as compared with control (11.6%; p < 0.01). Also, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 ug/ml heparin (68.4 and 73.6% fertilization percentage, respectively) as compared with control and 25 ug/ml heparin (13.5 and 41% cleavage rate; p < 0.01). It is concluded that Bali spermatozoa capacitated with 50-100 ug/ml heparin had significantly higher ability to improve capacitation status and acrosome reaction. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33605/1/33605.pdf Sarsaifi, Kazhal and Haron, Abd Wahid and Yusoff, Rosnina and Hani, Homayoun and Yimer, Nurhusien and Naing, Soh Win and Vejayan, Jaya and Othman, Abas Mazni (2012) Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations. In: International Conference on One Health & 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya, Malaysia. (pp. 67-68). |
| spellingShingle | Sarsaifi, Kazhal Haron, Abd Wahid Yusoff, Rosnina Hani, Homayoun Yimer, Nurhusien Naing, Soh Win Vejayan, Jaya Othman, Abas Mazni Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title | Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title_full | Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title_fullStr | Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title_full_unstemmed | Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title_short | Capacitation status of frozen thawed Bali bull (80S javanicus) spermatozoa with different heparin concentrations |
| title_sort | capacitation status of frozen thawed bali bull (80s javanicus) spermatozoa with different heparin concentrations |
| url | http://psasir.upm.edu.my/id/eprint/33605/ http://psasir.upm.edu.my/id/eprint/33605/1/33605.pdf |