Method for purifying serine protease recovered from a fruit source
A method for purifying proteases from a crude feedstock including the steps of providing a heating treatment to said crude feedstock at a plurality of time periods to produce a heat-treated feedstock in an aqueous two-phase system (ATPS), determining a proteolytic activity and a protein concentratio...
| Main Authors: | , , , , |
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| Format: | Patent |
| Published: |
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33153/ |
| Summary: | A method for purifying proteases from a crude feedstock including the steps of providing a heating treatment to said crude feedstock at a plurality of time periods to produce a heat-treated feedstock in an aqueous two-phase system (ATPS), determining a proteolytic activity and a protein concentration of the heat-treated feedstock, and recovering the protease from the heat-treated feedstock. The crude feedstock includes a source of fruit from which the proteases are recovered. |
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