Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes

The present invention relates to a method for early detection of Ganoderma boninense infection in oil palm and a method for indicating protection from Ganoderma boninense by a bio-control agent in a palm based on expression profile of genes associated with fatty acid (FA) biosynthetic pathway using...

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Main Authors: Abdullah, Siti Nor Akmar, Alizadeh, Fahimeh, Chong, Pei Pei, Yusuf, Umi Kalsom
Format: Patent
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/33126/
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author Abdullah, Siti Nor Akmar
Alizadeh, Fahimeh
Chong, Pei Pei
Yusuf, Umi Kalsom
author_facet Abdullah, Siti Nor Akmar
Alizadeh, Fahimeh
Chong, Pei Pei
Yusuf, Umi Kalsom
author_sort Abdullah, Siti Nor Akmar
building UPM Institutional Repository
collection Online Access
description The present invention relates to a method for early detection of Ganoderma boninense infection in oil palm and a method for indicating protection from Ganoderma boninense by a bio-control agent in a palm based on expression profile of genes associated with fatty acid (FA) biosynthetic pathway using roots and vegetative tissues comprising the step of: collecting a root or vegetative tissue sample, grounding the root or vegetative tissue sample and obtaining a powder, extracting the powder and obtaining a ribonucleic acid (RNA), designing at least a pair of primer based on the RNA, amplifying the pair of primer and obtaining an amplified nucleic acid and quantifying the amplified nucleic acid using a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and obtaining a gene expression profile transcript characterize in that the RNA is from a de novo fatty acid biosynthetic gene protein coding region.
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format Patent
id upm-33126
institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T09:18:04Z
publishDate 2011
recordtype eprints
repository_type Digital Repository
spelling upm-331262014-12-23T08:57:56Z http://psasir.upm.edu.my/id/eprint/33126/ Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes Abdullah, Siti Nor Akmar Alizadeh, Fahimeh Chong, Pei Pei Yusuf, Umi Kalsom The present invention relates to a method for early detection of Ganoderma boninense infection in oil palm and a method for indicating protection from Ganoderma boninense by a bio-control agent in a palm based on expression profile of genes associated with fatty acid (FA) biosynthetic pathway using roots and vegetative tissues comprising the step of: collecting a root or vegetative tissue sample, grounding the root or vegetative tissue sample and obtaining a powder, extracting the powder and obtaining a ribonucleic acid (RNA), designing at least a pair of primer based on the RNA, amplifying the pair of primer and obtaining an amplified nucleic acid and quantifying the amplified nucleic acid using a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and obtaining a gene expression profile transcript characterize in that the RNA is from a de novo fatty acid biosynthetic gene protein coding region. 2011-11-08 Patent NonPeerReviewed Siti Nor Akmar Abdullah (2011) Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes. PI2011005384.
spellingShingle Abdullah, Siti Nor Akmar
Alizadeh, Fahimeh
Chong, Pei Pei
Yusuf, Umi Kalsom
Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title_full Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title_fullStr Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title_full_unstemmed Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title_short Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
title_sort early detection of ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
url http://psasir.upm.edu.my/id/eprint/33126/