Multiplex assay for detection of Bario rice samples
The present invention provides a four-tube multiplex assay for distinguishing Bario rice from non-Bario rice varieties, said assay comprising the steps of running multiplex PCR with sample using one or more loci of Table 1 and distinguishing the Bario rice from non-Bario rice varieties on the basis...
| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/33118/ |
| Summary: | The present invention provides a four-tube multiplex assay for distinguishing Bario rice from non-Bario rice varieties, said assay comprising the steps of running multiplex PCR with sample using one or more loci of Table 1 and distinguishing the Bario rice from non-Bario rice varieties on the basis of varietal specific multiplex allele profile. The present invention also provides a kit for performing multiplex assay for distinguishing Bario rice from non-Bario rice varieties, which may comprise primers directed to SSR loci, appropriate reagents for PCR and a control DNA template of Bario rice. |
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