Thermostable proteases
New proteases are constantly isolated. Proteases, mainly the alkaline proteases are widely used in the detergent formulation. Thermostable alkaline proteases are of great interest in the detergent industry because these enzymes are stable and able to retain its activities over broad range of pH and...
| Main Authors: | , , , , |
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| Format: | Book Section |
| Language: | English |
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Nova Science Publishers
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/32974/ http://psasir.upm.edu.my/id/eprint/32974/1/Abstract-Thermostable%20proteases.pdf |
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| author | Ibrahim, Noor Azlina Leow, Adam Thean Chor Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Ibrahim, Noor Azlina Leow, Adam Thean Chor Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Ibrahim, Noor Azlina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | New proteases are constantly isolated. Proteases, mainly the alkaline proteases are widely used in the detergent formulation. Thermostable alkaline proteases are of great interest in the detergent industry because these enzymes are stable and able to retain its activities over broad range of pH and temperatures. Most thermostable alkaline proteases reported are from Bacillus spp. In this chapter, we introduce a new thermostable alkaline protease produced from Bacillus stearothermophilus strain F1. The protease production requirements by strain F1, the properties and characterization of this enzyme are discussed. The protease F1 gene was successfully cloned and expressed into E. coli XL1-Blue. The bacteriocin release protein(BRP)system was utilized; to release the recombinant F1 protease into the culture medium. The purified native and recombinant F1 protease showed a pH optimum of 9.0 and thermostabilities ranging from a half-life of 25 min at 90◦C to a half-life of 4 h at 85◦C. The optimumtemperatures were 85◦C and 80◦C respectively. |
| first_indexed | 2025-11-15T09:17:21Z |
| format | Book Section |
| id | upm-32974 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:17:21Z |
| publishDate | 2006 |
| publisher | Nova Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-329742016-09-26T04:07:32Z http://psasir.upm.edu.my/id/eprint/32974/ Thermostable proteases Ibrahim, Noor Azlina Leow, Adam Thean Chor Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran New proteases are constantly isolated. Proteases, mainly the alkaline proteases are widely used in the detergent formulation. Thermostable alkaline proteases are of great interest in the detergent industry because these enzymes are stable and able to retain its activities over broad range of pH and temperatures. Most thermostable alkaline proteases reported are from Bacillus spp. In this chapter, we introduce a new thermostable alkaline protease produced from Bacillus stearothermophilus strain F1. The protease production requirements by strain F1, the properties and characterization of this enzyme are discussed. The protease F1 gene was successfully cloned and expressed into E. coli XL1-Blue. The bacteriocin release protein(BRP)system was utilized; to release the recombinant F1 protease into the culture medium. The purified native and recombinant F1 protease showed a pH optimum of 9.0 and thermostabilities ranging from a half-life of 25 min at 90◦C to a half-life of 4 h at 85◦C. The optimumtemperatures were 85◦C and 80◦C respectively. Nova Science Publishers 2006 Book Section PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/32974/1/Abstract-Thermostable%20proteases.pdf Ibrahim, Noor Azlina and Leow, Adam Thean Chor and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2006) Thermostable proteases. In: New Lipases and Proteases. Nova Science Publishers, New York, pp. 77-93. ISBN 9781600210686 |
| spellingShingle | Ibrahim, Noor Azlina Leow, Adam Thean Chor Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Thermostable proteases |
| title | Thermostable proteases |
| title_full | Thermostable proteases |
| title_fullStr | Thermostable proteases |
| title_full_unstemmed | Thermostable proteases |
| title_short | Thermostable proteases |
| title_sort | thermostable proteases |
| url | http://psasir.upm.edu.my/id/eprint/32974/ http://psasir.upm.edu.my/id/eprint/32974/1/Abstract-Thermostable%20proteases.pdf |