Kajian Awal terhadap Sistem Pemilihan Somaklon CUi Yang Resistans kepada Colletotrichum capsici (Syd.) Butler & Bisby
Toxin treatment from the anthracnose pathogen, Colletotrichum capsici on chilli tissue culture was utilised as the selection mechanism for variants which are resistant to this pathogen. The crude toxin preparation was sterilised and then added to the MS medium at the concentration of 0, 10, 20, 30...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English Malay |
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Universiti Putra Malaysia Press
1994
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3259/ http://psasir.upm.edu.my/id/eprint/3259/1/Kajian_Awal_terhadap_Sistem_Pemilihan.pdf |
| _version_ | 1848839479946641408 |
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| author | Anuar, Nurina Ahmad, Ismail Hashim, Azizah |
| author_facet | Anuar, Nurina Ahmad, Ismail Hashim, Azizah |
| author_sort | Anuar, Nurina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Toxin treatment from the anthracnose pathogen, Colletotrichum capsici on chilli tissue culture was utilised as
the selection mechanism for variants which are resistant to this pathogen. The crude toxin preparation was
sterilised and then added to the MS medium at the concentration of 0, 10, 20, 30 or 40% (v/v). At concentrations
higher than 20 %, the toxin prevented the formation of calli from hypocotyl explants, and at 10% and 20 %,
the formation of calli was strongly inhibited. The calli which survived the toxin treatment did not regenerate.
Conversely, calli which were cultured on MS medium suplemented with VB juice or on the control MS medium,
were able to regenerate at 37.6% and 62.7%, respectively. Induction of resistant variants was attempted by
inoculating the calli with the konidia from the anthracnose pathogen. The antibiotic which had been extracted
from an antagonistic fungus, Chaetomium trilaterale, was added to the MS medium to limit the spread of the
pathogen to the medium from the chilli calli. From the antibiotic assay, the developement of the pathogen was
dependent on the antibiotic concentration used in the media. The minimum antibiotic concentration which
successfully prevented the growth of the fungal pathogen was 100 mg/ml. At that concentration, the fungus was
only observed on the calli but not on the medium. However, the calli turned brown after 2 weeks of inoculation.
In addition, the calli that survived became compacted and failed to regenerate. |
| first_indexed | 2025-11-15T07:12:07Z |
| format | Article |
| id | upm-3259 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English Malay |
| last_indexed | 2025-11-15T07:12:07Z |
| publishDate | 1994 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-32592013-05-27T07:06:48Z http://psasir.upm.edu.my/id/eprint/3259/ Kajian Awal terhadap Sistem Pemilihan Somaklon CUi Yang Resistans kepada Colletotrichum capsici (Syd.) Butler & Bisby Anuar, Nurina Ahmad, Ismail Hashim, Azizah Toxin treatment from the anthracnose pathogen, Colletotrichum capsici on chilli tissue culture was utilised as the selection mechanism for variants which are resistant to this pathogen. The crude toxin preparation was sterilised and then added to the MS medium at the concentration of 0, 10, 20, 30 or 40% (v/v). At concentrations higher than 20 %, the toxin prevented the formation of calli from hypocotyl explants, and at 10% and 20 %, the formation of calli was strongly inhibited. The calli which survived the toxin treatment did not regenerate. Conversely, calli which were cultured on MS medium suplemented with VB juice or on the control MS medium, were able to regenerate at 37.6% and 62.7%, respectively. Induction of resistant variants was attempted by inoculating the calli with the konidia from the anthracnose pathogen. The antibiotic which had been extracted from an antagonistic fungus, Chaetomium trilaterale, was added to the MS medium to limit the spread of the pathogen to the medium from the chilli calli. From the antibiotic assay, the developement of the pathogen was dependent on the antibiotic concentration used in the media. The minimum antibiotic concentration which successfully prevented the growth of the fungal pathogen was 100 mg/ml. At that concentration, the fungus was only observed on the calli but not on the medium. However, the calli turned brown after 2 weeks of inoculation. In addition, the calli that survived became compacted and failed to regenerate. Universiti Putra Malaysia Press 1994 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3259/1/Kajian_Awal_terhadap_Sistem_Pemilihan.pdf Anuar, Nurina and Ahmad, Ismail and Hashim, Azizah (1994) Kajian Awal terhadap Sistem Pemilihan Somaklon CUi Yang Resistans kepada Colletotrichum capsici (Syd.) Butler & Bisby. Pertanika Journal of Tropical Agricultural Science, 17 (3). pp. 213-218. ISSN 0126-6128 Malay |
| spellingShingle | Anuar, Nurina Ahmad, Ismail Hashim, Azizah Kajian Awal terhadap Sistem Pemilihan Somaklon CUi Yang Resistans kepada Colletotrichum capsici (Syd.) Butler & Bisby |
| title | Kajian Awal terhadap Sistem Pemilihan
Somaklon CUi Yang Resistans kepada
Colletotrichum capsici (Syd.) Butler & Bisby |
| title_full | Kajian Awal terhadap Sistem Pemilihan
Somaklon CUi Yang Resistans kepada
Colletotrichum capsici (Syd.) Butler & Bisby |
| title_fullStr | Kajian Awal terhadap Sistem Pemilihan
Somaklon CUi Yang Resistans kepada
Colletotrichum capsici (Syd.) Butler & Bisby |
| title_full_unstemmed | Kajian Awal terhadap Sistem Pemilihan
Somaklon CUi Yang Resistans kepada
Colletotrichum capsici (Syd.) Butler & Bisby |
| title_short | Kajian Awal terhadap Sistem Pemilihan
Somaklon CUi Yang Resistans kepada
Colletotrichum capsici (Syd.) Butler & Bisby |
| title_sort | kajian awal terhadap sistem pemilihan
somaklon cui yang resistans kepada
colletotrichum capsici (syd.) butler & bisby |
| url | http://psasir.upm.edu.my/id/eprint/3259/ http://psasir.upm.edu.my/id/eprint/3259/1/Kajian_Awal_terhadap_Sistem_Pemilihan.pdf |