Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions
The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent...
| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/32141/ |
| _version_ | 1848847135117672448 |
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| author | Ibrahim, Rashid Kamran, Kareh Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi |
| author_facet | Ibrahim, Rashid Kamran, Kareh Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi |
| author_sort | Ibrahim, Rashid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent cells are removed and the adherent cells are further expanded in a complete medium containing an increased amount of fetal bovine serum and after the expansion they are cultivated in an osteogenic inducing medium containing an increased amount of fetal bovine serum under standard conditions. The method is suitable also for bone marrow taken from a health-compromised mammal. |
| first_indexed | 2025-11-15T09:13:47Z |
| format | Patent |
| id | upm-32141 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:13:47Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-321412016-11-21T04:15:03Z http://psasir.upm.edu.my/id/eprint/32141/ Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions Ibrahim, Rashid Kamran, Kareh Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent cells are removed and the adherent cells are further expanded in a complete medium containing an increased amount of fetal bovine serum and after the expansion they are cultivated in an osteogenic inducing medium containing an increased amount of fetal bovine serum under standard conditions. The method is suitable also for bone marrow taken from a health-compromised mammal. 2010-09-08 Patent NonPeerReviewed Rashid Ibrahim (2010) Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions. PI2010700063. |
| spellingShingle | Ibrahim, Rashid Kamran, Kareh Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title | Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title_full | Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title_fullStr | Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title_full_unstemmed | Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title_short | Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| title_sort | method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions |
| url | http://psasir.upm.edu.my/id/eprint/32141/ |