Method for the purification of interferon.

The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and...

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Main Authors: Ling, Tau Chuan, Lin, Yu Kiat, Ooi, Chien Wei, Ariff, Arbakariya
Format: Patent
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/32048/
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author Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
author_facet Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
author_sort Ling, Tau Chuan
building UPM Institutional Repository
collection Online Access
description The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems.
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format Patent
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institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T09:13:23Z
publishDate 2011
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spelling upm-320482014-09-04T04:38:40Z http://psasir.upm.edu.my/id/eprint/32048/ Method for the purification of interferon. Ling, Tau Chuan Lin, Yu Kiat Ooi, Chien Wei Ariff, Arbakariya The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems. 2011-01-12 Patent NonPeerReviewed Ling Tau Chuan (2011) Method for the purification of interferon. PI2011000150.
spellingShingle Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
Method for the purification of interferon.
title Method for the purification of interferon.
title_full Method for the purification of interferon.
title_fullStr Method for the purification of interferon.
title_full_unstemmed Method for the purification of interferon.
title_short Method for the purification of interferon.
title_sort method for the purification of interferon.
url http://psasir.upm.edu.my/id/eprint/32048/