Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)

Spent hens (Rhode Island Red), aged 18 months and broilers (White Leghorn) aged 2 months were processed into a surimi-like material called Ayami. The processing method includes grinding the material twice through a 5mm grinder plate, followed by three steps of washing with ice cold water, after wh...

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Main Authors: Babji, Abdul Salam, Gna, Song Kee
Format: Article
Language:English
English
Published: Universiti Putra Malaysia Press 1994
Online Access:http://psasir.upm.edu.my/id/eprint/3200/
http://psasir.upm.edu.my/id/eprint/3200/1/Changes_in_Sarcoplasmic_and_Myofibrillar_Proteins_of_Spent_Hen.pdf
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author Babji, Abdul Salam
Gna, Song Kee
author_facet Babji, Abdul Salam
Gna, Song Kee
author_sort Babji, Abdul Salam
building UPM Institutional Repository
collection Online Access
description Spent hens (Rhode Island Red), aged 18 months and broilers (White Leghorn) aged 2 months were processed into a surimi-like material called Ayami. The processing method includes grinding the material twice through a 5mm grinder plate, followed by three steps of washing with ice cold water, after which the washed meat is mixed with cryoprotectants and stored in polythylene bags at - 15°C. Changes in sarcoplasmic and myofibrillar proteins during the processing stages were monitored using the snS-PAGE method. The electrophoretic pattern showed a decrease in intensity in the components of myofibrillar proteins after the first wash, with little changes after the second and third wash. For sarcoplasmic proteins, the intensity of components with molecular weights lower than 100,000 Dalton decreased, whereas the components with molecular weights higher than 100,000 Dalton increased. Washing has significant effects on the sarcoplasmatic and myofibrillar proteins of both spent hen and broiler meat. Such results could explain the gelation properties of surimi-like materials like Ayami.
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spelling upm-32002013-05-27T07:06:24Z http://psasir.upm.edu.my/id/eprint/3200/ Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami) Babji, Abdul Salam Gna, Song Kee Spent hens (Rhode Island Red), aged 18 months and broilers (White Leghorn) aged 2 months were processed into a surimi-like material called Ayami. The processing method includes grinding the material twice through a 5mm grinder plate, followed by three steps of washing with ice cold water, after which the washed meat is mixed with cryoprotectants and stored in polythylene bags at - 15°C. Changes in sarcoplasmic and myofibrillar proteins during the processing stages were monitored using the snS-PAGE method. The electrophoretic pattern showed a decrease in intensity in the components of myofibrillar proteins after the first wash, with little changes after the second and third wash. For sarcoplasmic proteins, the intensity of components with molecular weights lower than 100,000 Dalton decreased, whereas the components with molecular weights higher than 100,000 Dalton increased. Washing has significant effects on the sarcoplasmatic and myofibrillar proteins of both spent hen and broiler meat. Such results could explain the gelation properties of surimi-like materials like Ayami. Universiti Putra Malaysia Press 1994 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3200/1/Changes_in_Sarcoplasmic_and_Myofibrillar_Proteins_of_Spent_Hen.pdf Babji, Abdul Salam and Gna, Song Kee (1994) Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami). Pertanika Journal of Tropical Agricultural Science, 17 (2). pp. 117-123. ISSN 0126-6128 English
spellingShingle Babji, Abdul Salam
Gna, Song Kee
Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title_full Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title_fullStr Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title_full_unstemmed Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title_short Changes in Sarcoplasmic and Myofibrillar Proteins of Spent Hen and Broiler Meat during the Processing of Surimi-like Material (Ayami)
title_sort changes in sarcoplasmic and myofibrillar proteins of spent hen and broiler meat during the processing of surimi-like material (ayami)
url http://psasir.upm.edu.my/id/eprint/3200/
http://psasir.upm.edu.my/id/eprint/3200/1/Changes_in_Sarcoplasmic_and_Myofibrillar_Proteins_of_Spent_Hen.pdf