Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen
Display of the nucleocapsid protein (NP) of Nipah virus (NiV) on the hepatitis B core (HBc) particle enhances the antigenicity of the NP. In addition, the NP fused to the amino-teminus (NP401-532 HBc) and immunodominant loop of HBc (HBc79.80NP4O1'S31) was shown to be less sensitive to proteinas...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31947/ http://psasir.upm.edu.my/id/eprint/31947/1/31947.pdf |
| Summary: | Display of the nucleocapsid protein (NP) of Nipah virus (NiV) on the hepatitis B core (HBc) particle enhances the antigenicity of the NP. In addition, the NP fused to the amino-teminus (NP401-532 HBc) and immunodominant loop of HBc (HBc79.80NP4O1'S31) was shown to be less sensitive to proteinase K. Some of the proteinase K treated NP401.32HBc and HBc79.80NP,O].s32 remained intact and antigenic. These results indicated two important implications, (1) the use of HBc virus -like particle as a carrier platform for foreign epitopes, (2 ) the use of NP401.532HBc as a diagnostic reagent for NiV infection. |
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