A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from setp (a) with distilled water, (c) freeze-drying pellet o...
| Main Authors: | , , , , , , , , , , |
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| Format: | Patent |
| Published: |
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31547/ |
| _version_ | 1848846982286671872 |
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| author | Hassan, Mohd Ali Mohammadi, Mitra Abd Razak, Nor Asma Chong, Mei Ling Shirai, Yoshihito Wan Yunus, Wan Md Zin Abd. Aziz, Suraini Abdullah, Amirul Al-Ashraf Che Man, Hasfalina Anis, Siti Nor Syairah Phang, Lai Yee |
| author_facet | Hassan, Mohd Ali Mohammadi, Mitra Abd Razak, Nor Asma Chong, Mei Ling Shirai, Yoshihito Wan Yunus, Wan Md Zin Abd. Aziz, Suraini Abdullah, Amirul Al-Ashraf Che Man, Hasfalina Anis, Siti Nor Syairah Phang, Lai Yee |
| author_sort | Hassan, Mohd Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from setp (a) with distilled water, (c) freeze-drying pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM)from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f). |
| first_indexed | 2025-11-15T09:11:21Z |
| format | Patent |
| id | upm-31547 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:11:21Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-315472014-06-10T02:51:22Z http://psasir.upm.edu.my/id/eprint/31547/ A method for recovering an intracellular Polyhydroxyalkanoates (PHA). Hassan, Mohd Ali Mohammadi, Mitra Abd Razak, Nor Asma Chong, Mei Ling Shirai, Yoshihito Wan Yunus, Wan Md Zin Abd. Aziz, Suraini Abdullah, Amirul Al-Ashraf Che Man, Hasfalina Anis, Siti Nor Syairah Phang, Lai Yee The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from setp (a) with distilled water, (c) freeze-drying pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM)from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f). 2010-03-01 Patent NonPeerReviewed Mohd Ali Hassan (2010) A method for recovering an intracellular Polyhydroxyalkanoates (PHA). UNSPECIFIED. |
| spellingShingle | Hassan, Mohd Ali Mohammadi, Mitra Abd Razak, Nor Asma Chong, Mei Ling Shirai, Yoshihito Wan Yunus, Wan Md Zin Abd. Aziz, Suraini Abdullah, Amirul Al-Ashraf Che Man, Hasfalina Anis, Siti Nor Syairah Phang, Lai Yee A method for recovering an intracellular Polyhydroxyalkanoates (PHA). |
| title | A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
|
| title_full | A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
|
| title_fullStr | A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
|
| title_full_unstemmed | A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
|
| title_short | A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
|
| title_sort | method for recovering an intracellular polyhydroxyalkanoates (pha). |
| url | http://psasir.upm.edu.my/id/eprint/31547/ |