Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines
Temukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits. Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition p...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30726/ http://psasir.upm.edu.my/id/eprint/30726/1/antioxidant%20properties.pdf |
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| author | Tan, Bee Ling Mohd Esa, Norhaizan H. J., Suhaniza Lai, C. C. Saad, Norazalina Karim, Roselina |
| author_facet | Tan, Bee Ling Mohd Esa, Norhaizan H. J., Suhaniza Lai, C. C. Saad, Norazalina Karim, Roselina |
| author_sort | Tan, Bee Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Temukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits.
Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth
inhibition properties of temukut extract using colon cancer (HT-29), ovary cancer (Caov-3), and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the β-carotene bleaching assay, analysis of the DPPH radical scavenging capacity, and a FRAP assay. The
total phenolic compounds, oryzanol, vitamin E, and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the
cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells(p < 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to
temukut, (IC 50 = 55.30 μg/mL), whereas the highest sensitivity was observed in Caov-3 cells (IC 50=36.67 μg/
mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds, vitamin E, oryzanol, and phytic acid) in temukut was lower than that in rice bran, as has been previously reported, the
present study demonstrated temukut’s potential to inhibit the proliferation of HT-29, Caov-3, and HepG2 cells. |
| first_indexed | 2025-11-15T09:07:49Z |
| format | Article |
| id | upm-30726 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:07:49Z |
| publishDate | 2013 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-307262019-10-21T01:14:47Z http://psasir.upm.edu.my/id/eprint/30726/ Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines Tan, Bee Ling Mohd Esa, Norhaizan H. J., Suhaniza Lai, C. C. Saad, Norazalina Karim, Roselina Temukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits. Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition properties of temukut extract using colon cancer (HT-29), ovary cancer (Caov-3), and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the β-carotene bleaching assay, analysis of the DPPH radical scavenging capacity, and a FRAP assay. The total phenolic compounds, oryzanol, vitamin E, and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells(p < 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to temukut, (IC 50 = 55.30 μg/mL), whereas the highest sensitivity was observed in Caov-3 cells (IC 50=36.67 μg/ mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds, vitamin E, oryzanol, and phytic acid) in temukut was lower than that in rice bran, as has been previously reported, the present study demonstrated temukut’s potential to inhibit the proliferation of HT-29, Caov-3, and HepG2 cells. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30726/1/antioxidant%20properties.pdf Tan, Bee Ling and Mohd Esa, Norhaizan and H. J., Suhaniza and Lai, C. C. and Saad, Norazalina and Karim, Roselina (2013) Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines. International Food Research Journal, 20 (5). pp. 2117-2124. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/20%20(05)%202013/11%20IFRJ%2020%20(05)%202013%20Norhaizan%20094.pdf |
| spellingShingle | Tan, Bee Ling Mohd Esa, Norhaizan H. J., Suhaniza Lai, C. C. Saad, Norazalina Karim, Roselina Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title | Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title_full | Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title_fullStr | Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title_full_unstemmed | Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title_short | Antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| title_sort | antioxidant properties and antiproliferative effect of brewers' rice extract (temukut) on selected cancer cell lines |
| url | http://psasir.upm.edu.my/id/eprint/30726/ http://psasir.upm.edu.my/id/eprint/30726/ http://psasir.upm.edu.my/id/eprint/30726/1/antioxidant%20properties.pdf |