Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA)
Background: Phytic acid (PA) is a polyphosphorylated carbohydrate that can be found in high amounts in mostcereals, legumes, nut oil, seeds and soy beans. It has been suggested to play a significant role in inhibition of colorectal cancer. This study was conducted to investigate expression changes o...
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| Format: | Article |
| Language: | English |
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Asian Pacific Organization for Cancer Prevention
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30723/ http://psasir.upm.edu.my/id/eprint/30723/1/Suppression%20of%20%CE%B2.pdf |
| _version_ | 1848846759153893376 |
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| author | Saad, Norazalina Mohd Esa, Norhaizan Ithnin, Hairuszah |
| author_facet | Saad, Norazalina Mohd Esa, Norhaizan Ithnin, Hairuszah |
| author_sort | Saad, Norazalina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Phytic acid (PA) is a polyphosphorylated carbohydrate that can be found in high amounts in mostcereals, legumes, nut oil, seeds and soy beans. It has been suggested to play a significant role in inhibition of colorectal cancer. This study was conducted to investigate expression changes of β-catenin and cyclooxygenase-2 COX-2) and cell proliferation in the adenoma-carcinoma sequence after treatment with rice bran PA by immunocytochemistry. Materials and Methods: Seventy-two male Sprague-Dawley rats were divided into 6 equal groups with 12rats in each group. For cancer induction two intraperitoneal injections of azoxymethane AOM) were given at 15 mg/kg bodyweight over a 2-weeks period. During the post initiation phase, two different concentrations of PA, 0.2%(w/v) and 0.5% (w/v) were administered in the diet. Results: Results of ß-catenin, COX-2 expressions and cell proliferation of Ki-67 showed a significant contribution in colonic cancer progression. For ß-catenin and COX-2 expression, there was a significant difference between groups at p<0.05. With Ki-67, there was a statistically significant lowering the proliferating index as compared to AOM alone (p<0.05). A significant positive correlation (p=0.01) was noted between COX-2 expression and proliferation. Total ß-catenin also demonstrated a significant positive linear relationship with total COX-2 (p=0.044). Conclusions: This study indicated potential value of PA extracted from om rice bran in reducing colonic cancer risk in rats. |
| first_indexed | 2025-11-15T09:07:49Z |
| format | Article |
| id | upm-30723 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:07:49Z |
| publishDate | 2013 |
| publisher | Asian Pacific Organization for Cancer Prevention |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-307232016-02-05T07:42:02Z http://psasir.upm.edu.my/id/eprint/30723/ Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) Saad, Norazalina Mohd Esa, Norhaizan Ithnin, Hairuszah Background: Phytic acid (PA) is a polyphosphorylated carbohydrate that can be found in high amounts in mostcereals, legumes, nut oil, seeds and soy beans. It has been suggested to play a significant role in inhibition of colorectal cancer. This study was conducted to investigate expression changes of β-catenin and cyclooxygenase-2 COX-2) and cell proliferation in the adenoma-carcinoma sequence after treatment with rice bran PA by immunocytochemistry. Materials and Methods: Seventy-two male Sprague-Dawley rats were divided into 6 equal groups with 12rats in each group. For cancer induction two intraperitoneal injections of azoxymethane AOM) were given at 15 mg/kg bodyweight over a 2-weeks period. During the post initiation phase, two different concentrations of PA, 0.2%(w/v) and 0.5% (w/v) were administered in the diet. Results: Results of ß-catenin, COX-2 expressions and cell proliferation of Ki-67 showed a significant contribution in colonic cancer progression. For ß-catenin and COX-2 expression, there was a significant difference between groups at p<0.05. With Ki-67, there was a statistically significant lowering the proliferating index as compared to AOM alone (p<0.05). A significant positive correlation (p=0.01) was noted between COX-2 expression and proliferation. Total ß-catenin also demonstrated a significant positive linear relationship with total COX-2 (p=0.044). Conclusions: This study indicated potential value of PA extracted from om rice bran in reducing colonic cancer risk in rats. Asian Pacific Organization for Cancer Prevention 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30723/1/Suppression%20of%20%CE%B2.pdf Saad, Norazalina and Mohd Esa, Norhaizan and Ithnin, Hairuszah (2013) Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA). Asian Pacific Journal of Cancer Prevention, 14 (5). pp. 3093-3099. ISSN 1513-7368 http://www.apocpcontrol.org/page/apjcp_issues_view.php?pno=4518&gubun=p&s_search=&s_paper_vol=14&s_number33=5 10.7314/APJCP.2013.14.5.3093 |
| spellingShingle | Saad, Norazalina Mohd Esa, Norhaizan Ithnin, Hairuszah Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title | Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title_full | Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title_fullStr | Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title_full_unstemmed | Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title_short | Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA) |
| title_sort | suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (pa) |
| url | http://psasir.upm.edu.my/id/eprint/30723/ http://psasir.upm.edu.my/id/eprint/30723/ http://psasir.upm.edu.my/id/eprint/30723/ http://psasir.upm.edu.my/id/eprint/30723/1/Suppression%20of%20%CE%B2.pdf |