The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system

The aim of this study was to establish the effect of smaller molecular weight (0.5 and 1.0 kDa) on prebiotic efficacy and its putative sustainability in the human gut. The prebiotic effect of α-1,2 branched, 0.5 and 1 kDa dextrans were evaluated in faecal batch fermentations as compared with inulin....

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Main Authors: Sarbini, Shahrul Razid, Kolida, Sofia, Naeye, Thierry, Einerhand, Alexandra W., Gibson, Glenn R., Rastall, Robert A.
Format: Article
Language:English
Published: Elsevier 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30717/
http://psasir.upm.edu.my/id/eprint/30717/1/The%20prebiotic%20effect%20of%20%CE%B1.pdf
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author Sarbini, Shahrul Razid
Kolida, Sofia
Naeye, Thierry
Einerhand, Alexandra W.
Gibson, Glenn R.
Rastall, Robert A.
author_facet Sarbini, Shahrul Razid
Kolida, Sofia
Naeye, Thierry
Einerhand, Alexandra W.
Gibson, Glenn R.
Rastall, Robert A.
author_sort Sarbini, Shahrul Razid
building UPM Institutional Repository
collection Online Access
description The aim of this study was to establish the effect of smaller molecular weight (0.5 and 1.0 kDa) on prebiotic efficacy and its putative sustainability in the human gut. The prebiotic effect of α-1,2 branched, 0.5 and 1 kDa dextrans were evaluated in faecal batch fermentations as compared with inulin. Both dextrans induce similar selectivity towards Bifidobacterium sp., Lactobacillus/Enterococcus and Bacteroides/Prevotella, and producing similar concentrations of short chain fatty acids. However, the 0.5 kDa dextran was fermented faster than the 1 kDa dextran, where both produced lower amount of gas than inulin. The fermentation of 1 kDa dextran was further investigated in continuous gut models. The dextran increased Bifidobacterium and Roseburia sp. populations in the final vessel, while decreasing Clostridium histolyticum and Faecalibacterium prausnitzii. Overall, the α-1,2 branched, 1 kDa dextran induced selective effect on the gut microbiota and stimulated short chain fatty acids, indicating prebiotic sustainability in distal regions of the gut.
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spelling upm-307172016-02-17T01:58:34Z http://psasir.upm.edu.my/id/eprint/30717/ The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system Sarbini, Shahrul Razid Kolida, Sofia Naeye, Thierry Einerhand, Alexandra W. Gibson, Glenn R. Rastall, Robert A. The aim of this study was to establish the effect of smaller molecular weight (0.5 and 1.0 kDa) on prebiotic efficacy and its putative sustainability in the human gut. The prebiotic effect of α-1,2 branched, 0.5 and 1 kDa dextrans were evaluated in faecal batch fermentations as compared with inulin. Both dextrans induce similar selectivity towards Bifidobacterium sp., Lactobacillus/Enterococcus and Bacteroides/Prevotella, and producing similar concentrations of short chain fatty acids. However, the 0.5 kDa dextran was fermented faster than the 1 kDa dextran, where both produced lower amount of gas than inulin. The fermentation of 1 kDa dextran was further investigated in continuous gut models. The dextran increased Bifidobacterium and Roseburia sp. populations in the final vessel, while decreasing Clostridium histolyticum and Faecalibacterium prausnitzii. Overall, the α-1,2 branched, 1 kDa dextran induced selective effect on the gut microbiota and stimulated short chain fatty acids, indicating prebiotic sustainability in distal regions of the gut. Elsevier 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30717/1/The%20prebiotic%20effect%20of%20%CE%B1.pdf Sarbini, Shahrul Razid and Kolida, Sofia and Naeye, Thierry and Einerhand, Alexandra W. and Gibson, Glenn R. and Rastall, Robert A. (2013) The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system. Journal of Functional Foods, 5 (4). pp. 1938-1946. ISSN 1756-4646; ESSN: 2214-9414 10.1016/j.jff.2013.09.015
spellingShingle Sarbini, Shahrul Razid
Kolida, Sofia
Naeye, Thierry
Einerhand, Alexandra W.
Gibson, Glenn R.
Rastall, Robert A.
The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title_full The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title_fullStr The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title_full_unstemmed The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title_short The prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
title_sort prebiotic effect of α-1,2 branched, low molecular weight dextran in the batch and continuous faecal fermentation system
url http://psasir.upm.edu.my/id/eprint/30717/
http://psasir.upm.edu.my/id/eprint/30717/
http://psasir.upm.edu.my/id/eprint/30717/1/The%20prebiotic%20effect%20of%20%CE%B1.pdf