In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects
The fermentation selectivity of a commercial source of α-gluco- oligosaccharides (BioEcolians; Solabia) was investigated in vitro. Fermentation by faecal bacteria from four lean and four obese healthy adults was determined in anaerobic, pH-controlled faecal batch cultures. Inulin was used as a posit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30695/ http://psasir.upm.edu.my/id/eprint/30695/1/In%20vitro%20fermentation%20of%20commercial%20%CE%B1.pdf |
| _version_ | 1848846751448956928 |
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| author | Sarbini, Shahrul Razid Kolida, Sofia Gibson, Glenn R. Rastall, Robert A. |
| author_facet | Sarbini, Shahrul Razid Kolida, Sofia Gibson, Glenn R. Rastall, Robert A. |
| author_sort | Sarbini, Shahrul Razid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The fermentation selectivity of a commercial source of α-gluco- oligosaccharides (BioEcolians; Solabia) was investigated in vitro. Fermentation by faecal bacteria from four lean and four obese healthy adults was determined in anaerobic, pH-controlled faecal batch cultures. Inulin was used as a positive prebiotic control. Samples were obtained at 0, 10, 24 and 36 h for bacterial enumeration by fluorescent in situ hybridisation and SCFA analyses. Gas production during fermentation was investigated in non-pH-controlled batch cultures. α-Gluco-oligosaccharides significantly increased the Bifidobacterium sp. population compared with the control. Other bacterial groups enumerated were unaffected with the exception of an increase in the Bacteroides-Prevotella group and a decrease in Faecalibacterium prausnitzii on both α-gluco-oligosaccharides and inulin compared with baseline. An increase in acetate and propionate was seen on both substrates. The fermentation of α-gluco-oligosaccharides produced less total gas at a more gradual rate of production than inulin. Generally, substrates fermented with the obese microbiota produced similar results to the lean fermentation regarding bacteriology and metabolic activity. No significant difference at baseline (0 h) was detected between the lean and obese individuals in any of the faecal bacterial groups studied. |
| first_indexed | 2025-11-15T09:07:41Z |
| format | Article |
| id | upm-30695 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:07:41Z |
| publishDate | 2013 |
| publisher | Cambridge University Press |
| recordtype | eprints |
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| spelling | upm-306952016-02-05T01:09:51Z http://psasir.upm.edu.my/id/eprint/30695/ In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects Sarbini, Shahrul Razid Kolida, Sofia Gibson, Glenn R. Rastall, Robert A. The fermentation selectivity of a commercial source of α-gluco- oligosaccharides (BioEcolians; Solabia) was investigated in vitro. Fermentation by faecal bacteria from four lean and four obese healthy adults was determined in anaerobic, pH-controlled faecal batch cultures. Inulin was used as a positive prebiotic control. Samples were obtained at 0, 10, 24 and 36 h for bacterial enumeration by fluorescent in situ hybridisation and SCFA analyses. Gas production during fermentation was investigated in non-pH-controlled batch cultures. α-Gluco-oligosaccharides significantly increased the Bifidobacterium sp. population compared with the control. Other bacterial groups enumerated were unaffected with the exception of an increase in the Bacteroides-Prevotella group and a decrease in Faecalibacterium prausnitzii on both α-gluco-oligosaccharides and inulin compared with baseline. An increase in acetate and propionate was seen on both substrates. The fermentation of α-gluco-oligosaccharides produced less total gas at a more gradual rate of production than inulin. Generally, substrates fermented with the obese microbiota produced similar results to the lean fermentation regarding bacteriology and metabolic activity. No significant difference at baseline (0 h) was detected between the lean and obese individuals in any of the faecal bacterial groups studied. Cambridge University Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30695/1/In%20vitro%20fermentation%20of%20commercial%20%CE%B1.pdf Sarbini, Shahrul Razid and Kolida, Sofia and Gibson, Glenn R. and Rastall, Robert A. (2013) In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects. British Journal of Nutrition, 109 (11). pp. 1980-1989. ISSN 0007-1145; ESSN: 1475-2662 10.1017/S0007114512004205 |
| spellingShingle | Sarbini, Shahrul Razid Kolida, Sofia Gibson, Glenn R. Rastall, Robert A. In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title | In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title_full | In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title_fullStr | In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title_full_unstemmed | In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title_short | In vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| title_sort | in vitro fermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects |
| url | http://psasir.upm.edu.my/id/eprint/30695/ http://psasir.upm.edu.my/id/eprint/30695/ http://psasir.upm.edu.my/id/eprint/30695/1/In%20vitro%20fermentation%20of%20commercial%20%CE%B1.pdf |