Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system
The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30538/ http://psasir.upm.edu.my/id/eprint/30538/1/Simultaneous%20determination%20of%20aflatoxins.pdf http://psasir.upm.edu.my/id/eprint/30538/2/Simultaneous%20determination%20of%20aflatoxins.pdf |
| _version_ | 1848846705768792064 |
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| author | Rahmani, Atefeh Selamat, Jinap Khatib, Alfi Tan, Chin Ping |
| author_facet | Rahmani, Atefeh Selamat, Jinap Khatib, Alfi Tan, Chin Ping |
| author_sort | Rahmani, Atefeh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5, 10, and 100 ng/g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012, 0.05, and 0.5 ng/g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2 ng/g, correspondingly. |
| first_indexed | 2025-11-15T09:06:58Z |
| format | Article |
| id | upm-30538 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:06:58Z |
| publishDate | 2013 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-305382015-10-30T03:22:09Z http://psasir.upm.edu.my/id/eprint/30538/ Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system Rahmani, Atefeh Selamat, Jinap Khatib, Alfi Tan, Chin Ping The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5, 10, and 100 ng/g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012, 0.05, and 0.5 ng/g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2 ng/g, correspondingly. Taylor & Francis 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30538/1/Simultaneous%20determination%20of%20aflatoxins.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/30538/2/Simultaneous%20determination%20of%20aflatoxins.pdf Rahmani, Atefeh and Selamat, Jinap and Khatib, Alfi and Tan, Chin Ping (2013) Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system. Journal of Liquid Chromatography and Related Technologies, 36 (5). pp. 600-617. ISSN 1082-6076; ESSN: 1520-572X 10.1080/10826076.2012.670182 |
| spellingShingle | Rahmani, Atefeh Selamat, Jinap Khatib, Alfi Tan, Chin Ping Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title | Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title_full | Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title_fullStr | Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title_full_unstemmed | Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title_short | Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in cereals using a validated RP-HPLC method and phred derivatization system |
| title_sort | simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated rp-hplc method and phred derivatization system |
| url | http://psasir.upm.edu.my/id/eprint/30538/ http://psasir.upm.edu.my/id/eprint/30538/ http://psasir.upm.edu.my/id/eprint/30538/1/Simultaneous%20determination%20of%20aflatoxins.pdf http://psasir.upm.edu.my/id/eprint/30538/2/Simultaneous%20determination%20of%20aflatoxins.pdf |