Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli

An awareness of Escherichia coli as a foodborne pathogen and illness causing bacterium has been increased among consumers. Moreover, there is demand for natural product in order to reduce synthetic product that can cause toxic to the human. In this study, antibacterial activity, in term of MIC,...

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Main Authors: N. S., Zainin, Lau, K. Y., Zakaria, M., Radu, Son, Abdull Razis, Ahmad Faizal, Rukayadi, Yaya
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30524/
http://psasir.upm.edu.my/id/eprint/30524/1/30524.pdf
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author N. S., Zainin
Lau, K. Y.
Zakaria, M.
Radu, Son
Abdull Razis, Ahmad Faizal
Rukayadi, Yaya
author_facet N. S., Zainin
Lau, K. Y.
Zakaria, M.
Radu, Son
Abdull Razis, Ahmad Faizal
Rukayadi, Yaya
author_sort N. S., Zainin
building UPM Institutional Repository
collection Online Access
description An awareness of Escherichia coli as a foodborne pathogen and illness causing bacterium has been increased among consumers. Moreover, there is demand for natural product in order to reduce synthetic product that can cause toxic to the human. In this study, antibacterial activity, in term of MIC, MBC and killing-time curve of methanolic extract of Boesenbergia rotunda have been tested against a standard E. coli ATCC 25922 and two E. coli isolated from milk products using Clinical and Laboratory Standard Institute (CLSI) methods. The results show that B. rotunda extract was susceptible to all E. coli strains. The MIC and MBC values of B. rotunda extract against E. coli ranged 0.019 mg/mL 2.5 mg/mL and 0.039 mg/mL – 5.0 μg/mL, respectively. Killing-time curves were constructed at concentrations of 0x MIC, 1/2x MIC, 1x MIC, and 2x MIC. All E. coli strains can be killed with concentration of 2x MIC after 2 hours. The results show that B. rotunda extract has potential antibacterial activity against E. coli.
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spelling upm-305242017-01-24T01:54:44Z http://psasir.upm.edu.my/id/eprint/30524/ Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli N. S., Zainin Lau, K. Y. Zakaria, M. Radu, Son Abdull Razis, Ahmad Faizal Rukayadi, Yaya An awareness of Escherichia coli as a foodborne pathogen and illness causing bacterium has been increased among consumers. Moreover, there is demand for natural product in order to reduce synthetic product that can cause toxic to the human. In this study, antibacterial activity, in term of MIC, MBC and killing-time curve of methanolic extract of Boesenbergia rotunda have been tested against a standard E. coli ATCC 25922 and two E. coli isolated from milk products using Clinical and Laboratory Standard Institute (CLSI) methods. The results show that B. rotunda extract was susceptible to all E. coli strains. The MIC and MBC values of B. rotunda extract against E. coli ranged 0.019 mg/mL 2.5 mg/mL and 0.039 mg/mL – 5.0 μg/mL, respectively. Killing-time curves were constructed at concentrations of 0x MIC, 1/2x MIC, 1x MIC, and 2x MIC. All E. coli strains can be killed with concentration of 2x MIC after 2 hours. The results show that B. rotunda extract has potential antibacterial activity against E. coli. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30524/1/30524.pdf N. S., Zainin and Lau, K. Y. and Zakaria, M. and Radu, Son and Abdull Razis, Ahmad Faizal and Rukayadi, Yaya (2013) Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli. International Food Research Journal, 20 (6). pp. 3319-3323. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-20-2013.html
spellingShingle N. S., Zainin
Lau, K. Y.
Zakaria, M.
Radu, Son
Abdull Razis, Ahmad Faizal
Rukayadi, Yaya
Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title_full Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title_fullStr Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title_full_unstemmed Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title_short Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli
title_sort antibacterial activity of boesenbergia rotunda (l.) mansf. a. extract against escherichia coli
url http://psasir.upm.edu.my/id/eprint/30524/
http://psasir.upm.edu.my/id/eprint/30524/
http://psasir.upm.edu.my/id/eprint/30524/1/30524.pdf