Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.

Anthocyanins of Macedonian grape pomace from three varieties “Pinot noir”, “Merlot” and “Vranec” were isolated by high speed countercurrent chromatography. After purification of the fractions by means of preparative high performance liquid chromatography the structures of isolated pigments were eluc...

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Main Authors: Velickovska, Sanja Kostadinovic, Mirhosseini, Hamed, Bogeva, Elena
Format: Article
Language:English
Published: OMICS Publishing Group 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30508/
http://psasir.upm.edu.my/id/eprint/30508/1/Isolation%20of%20anthocyanins%20by%20highspeed%20countercurrent%20chromatography%20and%20application%20of%20the%20color%20activity%20concept%20to%20different%20varieties%20of%20red%20grape%20pomace%20from%20Macedonia..pdf
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author Velickovska, Sanja Kostadinovic
Mirhosseini, Hamed
Bogeva, Elena
author_facet Velickovska, Sanja Kostadinovic
Mirhosseini, Hamed
Bogeva, Elena
author_sort Velickovska, Sanja Kostadinovic
building UPM Institutional Repository
collection Online Access
description Anthocyanins of Macedonian grape pomace from three varieties “Pinot noir”, “Merlot” and “Vranec” were isolated by high speed countercurrent chromatography. After purification of the fractions by means of preparative high performance liquid chromatography the structures of isolated pigments were elucidated by electrospray ionization multiple mass spectrometry (ESI-MSn) and nuclear magnetic resonance (NMR) spectroscopy. The major anthocyanin malvidin-3-glucoside and the minor pigments delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside, and malvidin-3-p-coumarоyl-glucoside were isolated. The “Color activity concept” was applied and visual detection thresholds of isolated anthocyanins were determined. The results of the “color activity value” of the isolated pigments and their detection thresholds were in good agreement with the color shade of the different varieties of red grape pomace.
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spelling upm-305082015-10-07T04:03:54Z http://psasir.upm.edu.my/id/eprint/30508/ Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia. Velickovska, Sanja Kostadinovic Mirhosseini, Hamed Bogeva, Elena Anthocyanins of Macedonian grape pomace from three varieties “Pinot noir”, “Merlot” and “Vranec” were isolated by high speed countercurrent chromatography. After purification of the fractions by means of preparative high performance liquid chromatography the structures of isolated pigments were elucidated by electrospray ionization multiple mass spectrometry (ESI-MSn) and nuclear magnetic resonance (NMR) spectroscopy. The major anthocyanin malvidin-3-glucoside and the minor pigments delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside, and malvidin-3-p-coumarоyl-glucoside were isolated. The “Color activity concept” was applied and visual detection thresholds of isolated anthocyanins were determined. The results of the “color activity value” of the isolated pigments and their detection thresholds were in good agreement with the color shade of the different varieties of red grape pomace. OMICS Publishing Group 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30508/1/Isolation%20of%20anthocyanins%20by%20highspeed%20countercurrent%20chromatography%20and%20application%20of%20the%20color%20activity%20concept%20to%20different%20varieties%20of%20red%20grape%20pomace%20from%20Macedonia..pdf Velickovska, Sanja Kostadinovic and Mirhosseini, Hamed and Bogeva, Elena (2013) Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia. Journal of Nutrition and Food Sciences, 3 (6). pp. 243-250. ISSN 2155-9600 http://omicsonline.org/isolation-of-anthocyanins-by-highspeed-countercurrent-chromatography-and-application-of-the-color-activity-concept-to-different-varieties-of-red-grape-pomace-from-macedonia-2155-9600-1000243.php?aid=21019 10.4172/2155-9600.1000243
spellingShingle Velickovska, Sanja Kostadinovic
Mirhosseini, Hamed
Bogeva, Elena
Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title_full Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title_fullStr Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title_full_unstemmed Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title_short Isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from Macedonia.
title_sort isolation of anthocyanins by high-speed countercurrent chromatography and application of the color activity concept to different varieties of red grape pomace from macedonia.
url http://psasir.upm.edu.my/id/eprint/30508/
http://psasir.upm.edu.my/id/eprint/30508/
http://psasir.upm.edu.my/id/eprint/30508/
http://psasir.upm.edu.my/id/eprint/30508/1/Isolation%20of%20anthocyanins%20by%20highspeed%20countercurrent%20chromatography%20and%20application%20of%20the%20color%20activity%20concept%20to%20different%20varieties%20of%20red%20grape%20pomace%20from%20Macedonia..pdf