Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics

The use of Fourier transform infrared (FTIR) spectroscopy coupled with chemometric techniques to differentiate butter from beef fat (BF) was investigated. The spectral bands associated with butter, BF, and their mixtures were scanned, interpreted, and identified by relating them to those spectros...

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Main Authors: Ahmad Fadzlillah, Nurrulhidayah, Che Man, Yaakob, Abdul Rohman, Ismail, Amin, Mustafa, Shuhaimi, Khatib, Alfi
Format: Article
Language:English
English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30471/
http://psasir.upm.edu.my/id/eprint/30471/1/30471.pdf
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author Ahmad Fadzlillah, Nurrulhidayah
Che Man, Yaakob
Abdul Rohman,
Ismail, Amin
Mustafa, Shuhaimi
Khatib, Alfi
author_facet Ahmad Fadzlillah, Nurrulhidayah
Che Man, Yaakob
Abdul Rohman,
Ismail, Amin
Mustafa, Shuhaimi
Khatib, Alfi
author_sort Ahmad Fadzlillah, Nurrulhidayah
building UPM Institutional Repository
collection Online Access
description The use of Fourier transform infrared (FTIR) spectroscopy coupled with chemometric techniques to differentiate butter from beef fat (BF) was investigated. The spectral bands associated with butter, BF, and their mixtures were scanned, interpreted, and identified by relating them to those spectroscopically representative to pure butter and BF. For quantitative analysis, partial least square (PLS) regression was used to develop a calibration model at the selected fingerprint regions of 1500–1000 cm -1 , with the values of coefficient of determination (R 2 ) and root mean square error of calibration (RMSEC) are 0.999 and 0.89% (v/v), respectively. The PLS calibration model was subsequently used for the prediction of independent samples containing butter in the binary mixtures with BF. Using 6 principal components, root mean square error of prediction (RMSEP) is 2.42% (v/v). These results proved that FTIR spectroscopy in combination with multivariate calibration can be used for the detection and quantification of BF in butter formulation for authentication use.
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institution Universiti Putra Malaysia
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language English
English
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publishDate 2013
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
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spelling upm-304712015-06-01T02:58:23Z http://psasir.upm.edu.my/id/eprint/30471/ Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics Ahmad Fadzlillah, Nurrulhidayah Che Man, Yaakob Abdul Rohman, Ismail, Amin Mustafa, Shuhaimi Khatib, Alfi The use of Fourier transform infrared (FTIR) spectroscopy coupled with chemometric techniques to differentiate butter from beef fat (BF) was investigated. The spectral bands associated with butter, BF, and their mixtures were scanned, interpreted, and identified by relating them to those spectroscopically representative to pure butter and BF. For quantitative analysis, partial least square (PLS) regression was used to develop a calibration model at the selected fingerprint regions of 1500–1000 cm -1 , with the values of coefficient of determination (R 2 ) and root mean square error of calibration (RMSEC) are 0.999 and 0.89% (v/v), respectively. The PLS calibration model was subsequently used for the prediction of independent samples containing butter in the binary mixtures with BF. Using 6 principal components, root mean square error of prediction (RMSEP) is 2.42% (v/v). These results proved that FTIR spectroscopy in combination with multivariate calibration can be used for the detection and quantification of BF in butter formulation for authentication use. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30471/1/30471.pdf Ahmad Fadzlillah, Nurrulhidayah and Che Man, Yaakob and Abdul Rohman, and Ismail, Amin and Mustafa, Shuhaimi and Khatib, Alfi (2013) Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics. International Food Research Journal, 20 (3). pp. 1383-1388. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-3.html English
spellingShingle Ahmad Fadzlillah, Nurrulhidayah
Che Man, Yaakob
Abdul Rohman,
Ismail, Amin
Mustafa, Shuhaimi
Khatib, Alfi
Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title_full Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title_fullStr Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title_full_unstemmed Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title_short Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics
title_sort authentication analysis of butter from beef fat using fourier transform infrared (ftir) spectroscopy coupled with chemometrics
url http://psasir.upm.edu.my/id/eprint/30471/
http://psasir.upm.edu.my/id/eprint/30471/
http://psasir.upm.edu.my/id/eprint/30471/1/30471.pdf