Cultivation conditions for phytase production from recombinant escherichia coli DH5α
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Libertas Academica
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/30427/ http://psasir.upm.edu.my/id/eprint/30427/1/Cultivation%20conditions%20for%20phytase%20production%20from%20recombinant%20escherichia%20coli%20DH5%CE%B1.pdf |
| _version_ | 1848846673730600960 |
|---|---|
| author | Mohd Ariff, Rafidah Fitrianto, Anwar Abd Manap, Mohd Yazid Ideris, Aini Kasim, Azhar Suhairin, Afinah Meor Hussin, Anis Shobirin |
| author_facet | Mohd Ariff, Rafidah Fitrianto, Anwar Abd Manap, Mohd Yazid Ideris, Aini Kasim, Azhar Suhairin, Afinah Meor Hussin, Anis Shobirin |
| author_sort | Mohd Ariff, Rafidah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α. |
| first_indexed | 2025-11-15T09:06:27Z |
| format | Article |
| id | upm-30427 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:06:27Z |
| publishDate | 2013 |
| publisher | Libertas Academica |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-304272016-12-23T08:22:38Z http://psasir.upm.edu.my/id/eprint/30427/ Cultivation conditions for phytase production from recombinant escherichia coli DH5α Mohd Ariff, Rafidah Fitrianto, Anwar Abd Manap, Mohd Yazid Ideris, Aini Kasim, Azhar Suhairin, Afinah Meor Hussin, Anis Shobirin Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α. Libertas Academica 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30427/1/Cultivation%20conditions%20for%20phytase%20production%20from%20recombinant%20escherichia%20coli%20DH5%CE%B1.pdf Mohd Ariff, Rafidah and Fitrianto, Anwar and Abd Manap, Mohd Yazid and Ideris, Aini and Kasim, Azhar and Suhairin, Afinah and Meor Hussin, Anis Shobirin (2013) Cultivation conditions for phytase production from recombinant escherichia coli DH5α. Microbiology Insights, 6. pp. 17-28. ISSN 1178-6361 http://www.la-press.com/cultivation-conditions-for-phytase-production-from-recombinant-escheri-article-a3622 10.4137/MBI.S10402 |
| spellingShingle | Mohd Ariff, Rafidah Fitrianto, Anwar Abd Manap, Mohd Yazid Ideris, Aini Kasim, Azhar Suhairin, Afinah Meor Hussin, Anis Shobirin Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title | Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title_full | Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title_fullStr | Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title_full_unstemmed | Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title_short | Cultivation conditions for phytase production from recombinant escherichia coli DH5α |
| title_sort | cultivation conditions for phytase production from recombinant escherichia coli dh5α |
| url | http://psasir.upm.edu.my/id/eprint/30427/ http://psasir.upm.edu.my/id/eprint/30427/ http://psasir.upm.edu.my/id/eprint/30427/ http://psasir.upm.edu.my/id/eprint/30427/1/Cultivation%20conditions%20for%20phytase%20production%20from%20recombinant%20escherichia%20coli%20DH5%CE%B1.pdf |