A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata

Malaysia is one of the richest in its biodiversity in the world. There are not less than 12,000 plants species in its rain forest. The aim of this study is a continuous investigation for medicinal plants in Malaysia, especially the chemical constituents that are poses significant activities, i.e. an...

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Main Authors: Peh, Tian Hai, Lim, Gin Keat, Yap, Taufiq Yun Hin, Ee, Gwendoline Cheng Lian, Rahmani, Mawardi, Md. Sukari, Mohd Aspollah
Format: Article
Language:English
Published: Borderless Science Publishing 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30352/
http://psasir.upm.edu.my/id/eprint/30352/1/A%20new%20cytotoxic%20carbazole%20alkaloid%20isolated%20from%20the%20stem%20bark%20of%20Malaysian%20Clausena%20excavata.pdf
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author Peh, Tian Hai
Lim, Gin Keat
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
Rahmani, Mawardi
Md. Sukari, Mohd Aspollah
author_facet Peh, Tian Hai
Lim, Gin Keat
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
Rahmani, Mawardi
Md. Sukari, Mohd Aspollah
author_sort Peh, Tian Hai
building UPM Institutional Repository
collection Online Access
description Malaysia is one of the richest in its biodiversity in the world. There are not less than 12,000 plants species in its rain forest. The aim of this study is a continuous investigation for medicinal plants in Malaysia, especially the chemical constituents that are poses significant activities, i.e. anti-cancer. Clausena excavata (Rutaceae) has been known as a very rich in carbazole alkaloids, coumarins and limonoids species. In the present study, one new carbazole alkaloid, 1,8-dihydroxy-3-formyl-4- prenylcarbazole (Clausine–TH), and two other known compounds, Clausenarin (coumarin) and Clausine–K (carbazole alkaloid), were isolated from the methanol extract of the stem bark of Clausena excavata, collected from Kedah, Malaysia. Structures of these compounds were confirmed by various spectroscopic analyses including GC-MS, NMRs, and FTIR. All pure compounds isolated were tested for their cytotoxicity against CEM-SS cell lines, with the Clausine–TH and Clausine–K gave a very strong activity with an IC50 value of 2.1 µg/mL and 5.1 µg/mL, respectively.
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institution Universiti Putra Malaysia
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publisher Borderless Science Publishing
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spelling upm-303522016-11-25T08:34:58Z http://psasir.upm.edu.my/id/eprint/30352/ A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata Peh, Tian Hai Lim, Gin Keat Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Rahmani, Mawardi Md. Sukari, Mohd Aspollah Malaysia is one of the richest in its biodiversity in the world. There are not less than 12,000 plants species in its rain forest. The aim of this study is a continuous investigation for medicinal plants in Malaysia, especially the chemical constituents that are poses significant activities, i.e. anti-cancer. Clausena excavata (Rutaceae) has been known as a very rich in carbazole alkaloids, coumarins and limonoids species. In the present study, one new carbazole alkaloid, 1,8-dihydroxy-3-formyl-4- prenylcarbazole (Clausine–TH), and two other known compounds, Clausenarin (coumarin) and Clausine–K (carbazole alkaloid), were isolated from the methanol extract of the stem bark of Clausena excavata, collected from Kedah, Malaysia. Structures of these compounds were confirmed by various spectroscopic analyses including GC-MS, NMRs, and FTIR. All pure compounds isolated were tested for their cytotoxicity against CEM-SS cell lines, with the Clausine–TH and Clausine–K gave a very strong activity with an IC50 value of 2.1 µg/mL and 5.1 µg/mL, respectively. Borderless Science Publishing 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30352/1/A%20new%20cytotoxic%20carbazole%20alkaloid%20isolated%20from%20the%20stem%20bark%20of%20Malaysian%20Clausena%20excavata.pdf Peh, Tian Hai and Lim, Gin Keat and Yap, Taufiq Yun Hin and Ee, Gwendoline Cheng Lian and Rahmani, Mawardi and Md. Sukari, Mohd Aspollah (2013) A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata. Canadian Chemical Transactions, 1 (3). pp. 165-172. ISSN 2291-6458; ESSN: 2291-6466 http://canchemtrans.ca/index.php/list-of-issues/2013-1-3
spellingShingle Peh, Tian Hai
Lim, Gin Keat
Yap, Taufiq Yun Hin
Ee, Gwendoline Cheng Lian
Rahmani, Mawardi
Md. Sukari, Mohd Aspollah
A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title_full A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title_fullStr A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title_full_unstemmed A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title_short A new cytotoxic carbazole alkaloid isolated from the stem bark of Malaysian Clausena excavata
title_sort new cytotoxic carbazole alkaloid isolated from the stem bark of malaysian clausena excavata
url http://psasir.upm.edu.my/id/eprint/30352/
http://psasir.upm.edu.my/id/eprint/30352/
http://psasir.upm.edu.my/id/eprint/30352/1/A%20new%20cytotoxic%20carbazole%20alkaloid%20isolated%20from%20the%20stem%20bark%20of%20Malaysian%20Clausena%20excavata.pdf