Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30240/ http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf |
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| author | Masoumi, Hamid Reza Fard Basri, Mahiran Kassim, Anuar Abdullah, Dzulkefly Kuang Abdollahi, Yadollah Abd Gani, Siti Salwa Rezaee, Malahat |
| author_facet | Masoumi, Hamid Reza Fard Basri, Mahiran Kassim, Anuar Abdullah, Dzulkefly Kuang Abdollahi, Yadollah Abd Gani, Siti Salwa Rezaee, Malahat |
| author_sort | Masoumi, Hamid Reza Fard |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process. |
| first_indexed | 2025-11-15T09:05:37Z |
| format | Article |
| id | upm-30240 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:05:37Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-302402015-10-08T07:53:36Z http://psasir.upm.edu.my/id/eprint/30240/ Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor Masoumi, Hamid Reza Fard Basri, Mahiran Kassim, Anuar Abdullah, Dzulkefly Kuang Abdollahi, Yadollah Abd Gani, Siti Salwa Rezaee, Malahat Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf Masoumi, Hamid Reza Fard and Basri, Mahiran and Kassim, Anuar and Abdullah, Dzulkefly Kuang and Abdollahi, Yadollah and Abd Gani, Siti Salwa and Rezaee, Malahat (2013) Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor. The Scientific World Journal, 2013. art. no. 962083. pp. 1-9. ISSN 2356-6140; ESSN: 1537-744X 10.1155/2013/962083 English |
| spellingShingle | Masoumi, Hamid Reza Fard Basri, Mahiran Kassim, Anuar Abdullah, Dzulkefly Kuang Abdollahi, Yadollah Abd Gani, Siti Salwa Rezaee, Malahat Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title | Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title_full | Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title_fullStr | Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title_full_unstemmed | Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title_short | Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| title_sort | statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
| url | http://psasir.upm.edu.my/id/eprint/30240/ http://psasir.upm.edu.my/id/eprint/30240/ http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf |