Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.

Background: The aim of the current study is to design a new nanocomposite for inducing cytotoxicity of doxorubicin and oxaliplatin toward MDA-MB231, MCF-7, and Caco2 cell lines. A hippuric acid (HA) zinc layered hydroxide (ZLH) nanocomposite was synthesized under an aqueous environment using H...

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Main Authors:	Hussein Al Ali, Samer Hassan, Al-Qubaisi, Mothanna, Hussein, Mohd Zobir, Ismail, Maznah, Bullo, Saifullah
Format:	Article
Language:	English English
Published:	Dove Medical Press 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/30041/ http://psasir.upm.edu.my/id/eprint/30041/1/Hippuric%20acid%20nanocomposite%20enhances%20doxorubicin%20and%20oxaliplatin.pdf

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author	Hussein Al Ali, Samer Hassan Al-Qubaisi, Mothanna Hussein, Mohd Zobir Ismail, Maznah Bullo, Saifullah
author_facet	Hussein Al Ali, Samer Hassan Al-Qubaisi, Mothanna Hussein, Mohd Zobir Ismail, Maznah Bullo, Saifullah
author_sort	Hussein Al Ali, Samer Hassan
building	UPM Institutional Repository
collection	Online Access
description	Background: The aim of the current study is to design a new nanocomposite for inducing cytotoxicity of doxorubicin and oxaliplatin toward MDA-MB231, MCF-7, and Caco2 cell lines. A hippuric acid (HA) zinc layered hydroxide (ZLH) nanocomposite was synthesized under an aqueous environment using HA and zinc oxide (ZnO) as the precursors. Methods: The hippuric acid nanocomposite (HAN) was prepared by the direct reaction of a HA solution with an aqueous suspension of ZnO. Results: The basal spacing of the nanocomposite was 21.3 Å, which is average of four harmonics at 2θ = 8.32°, 12.50°, 16.68°, and 20.84°. This result indicates that the hippurate anion was successfully intercalated into the interlayer space of ZLH. The combinations of HAN with chemotherapy (drugs) has inhibited the cell growth of the MDA-MB231, MCF-7, and Caco2 cancer cells when compared to drugs alone. An IC50 value for the combination of HAN with doxorubicin toward MCF-7 is 0.19 ± 0.15 µg/mL and toward MDA-MB231 is 0.13 ± 0.10 µg/mL. Similarly, the IC50 for the combination of HAN with oxaliplatin toward Caco2 is 0.24 ± 0.11 µg/mL. In the antiproliferative results, the equal combination of HAN (0.5 µg/mL) with doxorubicin (0.5 µg/mL) has reduced the cell proliferation in MCF-7 and MDA-MB- 231 cells into 37.3% and 17.6%, respectively after 24 hours. Similarly, the antiproliferation percentage for equal combination HAN with oxaliplatin (5.00 µg/mL) toward Caco2 is 72.7% after 24 hours. Conclusion: The resulting combination HAN with drugs has exhibited higher inhibition in cells growth in all cancer cell lines
first_indexed	2025-11-15T09:04:45Z
format	Article
id	upm-30041
institution	Universiti Putra Malaysia
institution_category	Local University
language	English English
last_indexed	2025-11-15T09:04:45Z
publishDate	2013
publisher	Dove Medical Press
recordtype	eprints
repository_type	Digital Repository
spelling	upm-300412015-09-11T01:46:50Z http://psasir.upm.edu.my/id/eprint/30041/ Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines. Hussein Al Ali, Samer Hassan Al-Qubaisi, Mothanna Hussein, Mohd Zobir Ismail, Maznah Bullo, Saifullah Background: The aim of the current study is to design a new nanocomposite for inducing cytotoxicity of doxorubicin and oxaliplatin toward MDA-MB231, MCF-7, and Caco2 cell lines. A hippuric acid (HA) zinc layered hydroxide (ZLH) nanocomposite was synthesized under an aqueous environment using HA and zinc oxide (ZnO) as the precursors. Methods: The hippuric acid nanocomposite (HAN) was prepared by the direct reaction of a HA solution with an aqueous suspension of ZnO. Results: The basal spacing of the nanocomposite was 21.3 Å, which is average of four harmonics at 2θ = 8.32°, 12.50°, 16.68°, and 20.84°. This result indicates that the hippurate anion was successfully intercalated into the interlayer space of ZLH. The combinations of HAN with chemotherapy (drugs) has inhibited the cell growth of the MDA-MB231, MCF-7, and Caco2 cancer cells when compared to drugs alone. An IC50 value for the combination of HAN with doxorubicin toward MCF-7 is 0.19 ± 0.15 µg/mL and toward MDA-MB231 is 0.13 ± 0.10 µg/mL. Similarly, the IC50 for the combination of HAN with oxaliplatin toward Caco2 is 0.24 ± 0.11 µg/mL. In the antiproliferative results, the equal combination of HAN (0.5 µg/mL) with doxorubicin (0.5 µg/mL) has reduced the cell proliferation in MCF-7 and MDA-MB- 231 cells into 37.3% and 17.6%, respectively after 24 hours. Similarly, the antiproliferation percentage for equal combination HAN with oxaliplatin (5.00 µg/mL) toward Caco2 is 72.7% after 24 hours. Conclusion: The resulting combination HAN with drugs has exhibited higher inhibition in cells growth in all cancer cell lines Dove Medical Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30041/1/Hippuric%20acid%20nanocomposite%20enhances%20doxorubicin%20and%20oxaliplatin.pdf Hussein Al Ali, Samer Hassan and Al-Qubaisi, Mothanna and Hussein, Mohd Zobir and Ismail, Maznah and Bullo, Saifullah (2013) Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines. Drug Design, Development and Therapy, 7. pp. 25-31. ISSN 1177-8881 https://www.dovepress.com/drug-design-development-and-therapy-i741-j19 10.2147/DDDT.S37070 English
spellingShingle	Hussein Al Ali, Samer Hassan Al-Qubaisi, Mothanna Hussein, Mohd Zobir Ismail, Maznah Bullo, Saifullah Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title	Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title_full	Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title_fullStr	Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title_full_unstemmed	Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title_short	Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.
title_sort	hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in mda-mb231, mcf-7 and caco2 cell lines.
url	http://psasir.upm.edu.my/id/eprint/30041/ http://psasir.upm.edu.my/id/eprint/30041/ http://psasir.upm.edu.my/id/eprint/30041/ http://psasir.upm.edu.my/id/eprint/30041/1/Hippuric%20acid%20nanocomposite%20enhances%20doxorubicin%20and%20oxaliplatin.pdf

Hippuric acid nanocomposite enhances doxorubicin and oxaliplatin-induced cytotoxicity in MDA-MB231, MCF-7 and Caco2 cell lines.

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