A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30040/ http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf |
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| author | Anasamy, Theebaa Abdul, Ahmad Bustamam Sukari, Mohd Aspollah Abdelwahab, Siddig Ibrahim Mohan, Syam Kamalidehghan, Behnam Azid, Mohd Zulkhairi Muhammad Nadzri, Nabilah Andas, A. Reenaa Joys Beng, Ng Kuan A. Hadi, A. Hamid Rahman, Heshu Sulaiman |
| author_facet | Anasamy, Theebaa Abdul, Ahmad Bustamam Sukari, Mohd Aspollah Abdelwahab, Siddig Ibrahim Mohan, Syam Kamalidehghan, Behnam Azid, Mohd Zulkhairi Muhammad Nadzri, Nabilah Andas, A. Reenaa Joys Beng, Ng Kuan A. Hadi, A. Hamid Rahman, Heshu Sulaiman |
| author_sort | Anasamy, Theebaa |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered. Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an ICvalue of 7.11 ± 0.240 g/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC> 50 g/mL). Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis. ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells. Decline of mitochondrial membrane potential was also determined qualitatively. In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID. These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction. |
| first_indexed | 2025-11-15T09:04:44Z |
| format | Article |
| id | upm-30040 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:04:44Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-300402016-02-12T08:13:18Z http://psasir.upm.edu.my/id/eprint/30040/ A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway Anasamy, Theebaa Abdul, Ahmad Bustamam Sukari, Mohd Aspollah Abdelwahab, Siddig Ibrahim Mohan, Syam Kamalidehghan, Behnam Azid, Mohd Zulkhairi Muhammad Nadzri, Nabilah Andas, A. Reenaa Joys Beng, Ng Kuan A. Hadi, A. Hamid Rahman, Heshu Sulaiman The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered. Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an ICvalue of 7.11 ± 0.240 g/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC> 50 g/mL). Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis. ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells. Decline of mitochondrial membrane potential was also determined qualitatively. In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID. These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf Anasamy, Theebaa and Abdul, Ahmad Bustamam and Sukari, Mohd Aspollah and Abdelwahab, Siddig Ibrahim and Mohan, Syam and Kamalidehghan, Behnam and Azid, Mohd Zulkhairi and Muhammad Nadzri, Nabilah and Andas, A. Reenaa Joys and Beng, Ng Kuan and A. Hadi, A. Hamid and Rahman, Heshu Sulaiman (2013) A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway. Evidence-Based Complementary and Alternative Medicine, 2013 (939810). pp. 1-14. ISSN 1741-427X; ESSN: 1741-4288 http://dx.doi.org/10.1155/2013/939810 10.1155/2013/939810 |
| spellingShingle | Anasamy, Theebaa Abdul, Ahmad Bustamam Sukari, Mohd Aspollah Abdelwahab, Siddig Ibrahim Mohan, Syam Kamalidehghan, Behnam Azid, Mohd Zulkhairi Muhammad Nadzri, Nabilah Andas, A. Reenaa Joys Beng, Ng Kuan A. Hadi, A. Hamid Rahman, Heshu Sulaiman A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title | A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title_full | A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title_fullStr | A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title_full_unstemmed | A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title_short | A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| title_sort | phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(e)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in t-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway |
| url | http://psasir.upm.edu.my/id/eprint/30040/ http://psasir.upm.edu.my/id/eprint/30040/ http://psasir.upm.edu.my/id/eprint/30040/ http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf |