Characterization of wound responsive genes in Aquilaria malaccensis.
We report on the isolation and characterization of several genes responsive to wounding in the tropical endangered tree Aquilaria malaccensis. Wounding triggers the formation of a fragrant substance inside the tree stem. Deduced amino acid of the cloned sequences exhibited sequence similarities to t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29989/ http://psasir.upm.edu.my/id/eprint/29989/1/Characterization%20of%20wound%20responsive%20genes%20in%20Aquilaria%20malaccensis.pdf |
| _version_ | 1848846551653285888 |
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| author | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi |
| author_facet | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi |
| author_sort | Wong, Mun Theng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We report on the isolation and characterization of several genes responsive to wounding in the tropical endangered tree Aquilaria malaccensis. Wounding triggers the formation of a fragrant substance inside the tree stem. Deduced amino acid of the cloned sequences exhibited sequence similarities to their respective homologs: transcription factors of the WRKY gene family (AmWRKY) and β-1,3-glucanase (AmGLU). A homolog to phenylalanine ammonia-lyase (AmPAL) from previous work was also included. All cDNA sequences were of partial lengths. We studied their expression profiles in a wounding-stress experiment. Mechanical wounding induces AmWRKY in an early response to wounding (3 h), and elevates AmPAL and AmGLU expressions after 16 h. It is possible that AmWRKY mediates early wounding response while AmPAL mediates response to fungal infection by co-inducing AmGLU. Their homologs in other plants are known to inhibit fungal growth. Our data provide the first insight into the mechanisms of wounding responses in Aquilaria. |
| first_indexed | 2025-11-15T09:04:31Z |
| format | Article |
| id | upm-29989 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:04:31Z |
| publishDate | 2013 |
| publisher | Springer Verlag |
| recordtype | eprints |
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| spelling | upm-299892015-10-09T01:02:53Z http://psasir.upm.edu.my/id/eprint/29989/ Characterization of wound responsive genes in Aquilaria malaccensis. Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi We report on the isolation and characterization of several genes responsive to wounding in the tropical endangered tree Aquilaria malaccensis. Wounding triggers the formation of a fragrant substance inside the tree stem. Deduced amino acid of the cloned sequences exhibited sequence similarities to their respective homologs: transcription factors of the WRKY gene family (AmWRKY) and β-1,3-glucanase (AmGLU). A homolog to phenylalanine ammonia-lyase (AmPAL) from previous work was also included. All cDNA sequences were of partial lengths. We studied their expression profiles in a wounding-stress experiment. Mechanical wounding induces AmWRKY in an early response to wounding (3 h), and elevates AmPAL and AmGLU expressions after 16 h. It is possible that AmWRKY mediates early wounding response while AmPAL mediates response to fungal infection by co-inducing AmGLU. Their homologs in other plants are known to inhibit fungal growth. Our data provide the first insight into the mechanisms of wounding responses in Aquilaria. Springer Verlag 2013-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29989/1/Characterization%20of%20wound%20responsive%20genes%20in%20Aquilaria%20malaccensis.pdf Wong, Mun Theng and Siah, Chai Har and Qamaruz Zaman, Faridah and Mohamed, Rozi (2013) Characterization of wound responsive genes in Aquilaria malaccensis. Journal of Plant Biochemistry and Biotechnology, 22 (2). pp. 168-175. ISSN 0971-7811; ESSN: 0974-1275 10.1007/s13562-012-0144-z English |
| spellingShingle | Wong, Mun Theng Siah, Chai Har Qamaruz Zaman, Faridah Mohamed, Rozi Characterization of wound responsive genes in Aquilaria malaccensis. |
| title | Characterization of wound responsive genes in Aquilaria malaccensis. |
| title_full | Characterization of wound responsive genes in Aquilaria malaccensis. |
| title_fullStr | Characterization of wound responsive genes in Aquilaria malaccensis. |
| title_full_unstemmed | Characterization of wound responsive genes in Aquilaria malaccensis. |
| title_short | Characterization of wound responsive genes in Aquilaria malaccensis. |
| title_sort | characterization of wound responsive genes in aquilaria malaccensis. |
| url | http://psasir.upm.edu.my/id/eprint/29989/ http://psasir.upm.edu.my/id/eprint/29989/ http://psasir.upm.edu.my/id/eprint/29989/1/Characterization%20of%20wound%20responsive%20genes%20in%20Aquilaria%20malaccensis.pdf |