Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma.
The aim of this study was to search for bioactive natural products from medicinal plants targeting vasorelaxant activity and we found the methanol extract from bark of Tabernaemontana dichotoma showed vasorelaxant activity on rat aorta. We isolated eight indole alkaloids including 10-methoxyalstoner...
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| Format: | Article |
| Language: | English |
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Springer Verlag
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29981/ http://psasir.upm.edu.my/id/eprint/29981/1/Vasorelaxant%20activity%20of%20indole%20alkaloids%20from%20Tabernaemontana%20dichotoma.pdf |
| _version_ | 1848846549189132288 |
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| author | Zaima, Kazumasa Koga, Ikumi Iwasawa, Nobuhide Hosoya, Takahiro Hirasawa, Yusuke Kaneda, Toshio Ismail, Intan Safinar Lajis, Nordin Morita, Hiroshi |
| author_facet | Zaima, Kazumasa Koga, Ikumi Iwasawa, Nobuhide Hosoya, Takahiro Hirasawa, Yusuke Kaneda, Toshio Ismail, Intan Safinar Lajis, Nordin Morita, Hiroshi |
| author_sort | Zaima, Kazumasa |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to search for bioactive natural products from medicinal plants targeting vasorelaxant activity and we found the methanol extract from bark of Tabernaemontana dichotoma showed vasorelaxant activity on rat aorta. We isolated eight indole alkaloids including 10-methoxyalstonerine (1), a new macroline type indole alkaloid, from bark of T. dichotoma. These were respectively identified as 10-methoxyaffinisine (2), lochnerine (3), cathafoline (4), (−)-alstonerine (5), 19,20-dehydro-10-methoxytalcarpine (6), alstonisine (7), and alstonal (8) based on spectroscopic analysis. Among them, sarpagine type (2 and 3), akuammiline type (4), and macroline oxindole type (7 and 8) showed potent vasorelaxant activity. Mechanism of action on vasorelaxant activity of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) was clarified. Effects of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) were partially mediated the NO release from endothelial cells. Furthermore, 10-methoxyaffinisine (2) and alstonisine (7) attribute to the inhibitory effect of VDC and ROC, and cathafoline (4) have inhibitory effect on Ca2+ influx via ROC. In addition, 10-methoxyaffinisine (2) as a major compound from bark of T. dichotoma showed hypotensive effect on normotensive rats in vivo. |
| first_indexed | 2025-11-15T09:04:28Z |
| format | Article |
| id | upm-29981 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:04:28Z |
| publishDate | 2013 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-299812015-11-17T01:24:35Z http://psasir.upm.edu.my/id/eprint/29981/ Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. Zaima, Kazumasa Koga, Ikumi Iwasawa, Nobuhide Hosoya, Takahiro Hirasawa, Yusuke Kaneda, Toshio Ismail, Intan Safinar Lajis, Nordin Morita, Hiroshi The aim of this study was to search for bioactive natural products from medicinal plants targeting vasorelaxant activity and we found the methanol extract from bark of Tabernaemontana dichotoma showed vasorelaxant activity on rat aorta. We isolated eight indole alkaloids including 10-methoxyalstonerine (1), a new macroline type indole alkaloid, from bark of T. dichotoma. These were respectively identified as 10-methoxyaffinisine (2), lochnerine (3), cathafoline (4), (−)-alstonerine (5), 19,20-dehydro-10-methoxytalcarpine (6), alstonisine (7), and alstonal (8) based on spectroscopic analysis. Among them, sarpagine type (2 and 3), akuammiline type (4), and macroline oxindole type (7 and 8) showed potent vasorelaxant activity. Mechanism of action on vasorelaxant activity of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) was clarified. Effects of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) were partially mediated the NO release from endothelial cells. Furthermore, 10-methoxyaffinisine (2) and alstonisine (7) attribute to the inhibitory effect of VDC and ROC, and cathafoline (4) have inhibitory effect on Ca2+ influx via ROC. In addition, 10-methoxyaffinisine (2) as a major compound from bark of T. dichotoma showed hypotensive effect on normotensive rats in vivo. Springer Verlag 2013-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29981/1/Vasorelaxant%20activity%20of%20indole%20alkaloids%20from%20Tabernaemontana%20dichotoma.pdf Zaima, Kazumasa and Koga, Ikumi and Iwasawa, Nobuhide and Hosoya, Takahiro and Hirasawa, Yusuke and Kaneda, Toshio and Ismail, Intan Safinar and Lajis, Nordin and Morita, Hiroshi (2013) Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. Journal of Natural Medicines, 67 (1). pp. 9-16. ISSN 1340-3443; ESSN : 1861-0293 10.1007/s11418-012-0638-y |
| spellingShingle | Zaima, Kazumasa Koga, Ikumi Iwasawa, Nobuhide Hosoya, Takahiro Hirasawa, Yusuke Kaneda, Toshio Ismail, Intan Safinar Lajis, Nordin Morita, Hiroshi Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title | Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title_full | Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title_fullStr | Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title_full_unstemmed | Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title_short | Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma. |
| title_sort | vasorelaxant activity of indole alkaloids from tabernaemontana dichotoma. |
| url | http://psasir.upm.edu.my/id/eprint/29981/ http://psasir.upm.edu.my/id/eprint/29981/ http://psasir.upm.edu.my/id/eprint/29981/1/Vasorelaxant%20activity%20of%20indole%20alkaloids%20from%20Tabernaemontana%20dichotoma.pdf |