Molecular detection and chracterization of infectious bronchitis virus from Libya.

Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and...

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Main Authors: Kammon, Abdulwahab, Tan, Sheau Wei, Omar, Abdul Rahman, Dayhum, Abdunaser, Eldghayes, Ibrahim, Sharif, Monier
Format: Article
Language:English
English
Published: World Academy of Science, Engineering and Technology 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29928/
http://psasir.upm.edu.my/id/eprint/29928/1/Molecular%20detection%20and%20chracterization%20of%20infectious%20bronchitis%20virus%20from%20Libya.pdf
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author Kammon, Abdulwahab
Tan, Sheau Wei
Omar, Abdul Rahman
Dayhum, Abdunaser
Eldghayes, Ibrahim
Sharif, Monier
author_facet Kammon, Abdulwahab
Tan, Sheau Wei
Omar, Abdul Rahman
Dayhum, Abdunaser
Eldghayes, Ibrahim
Sharif, Monier
author_sort Kammon, Abdulwahab
building UPM Institutional Repository
collection Online Access
description Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and cloacal swabs were analyzed for several poultry viruses. IBV was detected using SYBR Green I real-time PCR detection based on the nucleocapsid (N) gene. Sequence analysis of the partial N gene indicated high similarity (~ 94%) to IBV strain 3382/06 that was isolated from Taiwan. Even though the IBV strain 3382/06 is more similar to that of the Mass type H120, the isolate has been implicated associated with intertypic recombinant of 3 putative parental IBV strains namely H120, Taiwan strain 1171/92 and China strain CK/CH/LDL/97I. Complete sequencing and antigenicity studies of the Libya IBV strains are currently underway to determine the evolution of the virus and its importance in vaccine induced immunity. In this paper we documented for the first time the presence of possibly variant IBV strain from Libya which required dramatic change in vaccination program.
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spelling upm-299282015-09-18T06:37:01Z http://psasir.upm.edu.my/id/eprint/29928/ Molecular detection and chracterization of infectious bronchitis virus from Libya. Kammon, Abdulwahab Tan, Sheau Wei Omar, Abdul Rahman Dayhum, Abdunaser Eldghayes, Ibrahim Sharif, Monier Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and cloacal swabs were analyzed for several poultry viruses. IBV was detected using SYBR Green I real-time PCR detection based on the nucleocapsid (N) gene. Sequence analysis of the partial N gene indicated high similarity (~ 94%) to IBV strain 3382/06 that was isolated from Taiwan. Even though the IBV strain 3382/06 is more similar to that of the Mass type H120, the isolate has been implicated associated with intertypic recombinant of 3 putative parental IBV strains namely H120, Taiwan strain 1171/92 and China strain CK/CH/LDL/97I. Complete sequencing and antigenicity studies of the Libya IBV strains are currently underway to determine the evolution of the virus and its importance in vaccine induced immunity. In this paper we documented for the first time the presence of possibly variant IBV strain from Libya which required dramatic change in vaccination program. World Academy of Science, Engineering and Technology 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29928/1/Molecular%20detection%20and%20chracterization%20of%20infectious%20bronchitis%20virus%20from%20Libya.pdf Kammon, Abdulwahab and Tan, Sheau Wei and Omar, Abdul Rahman and Dayhum, Abdunaser and Eldghayes, Ibrahim and Sharif, Monier (2013) Molecular detection and chracterization of infectious bronchitis virus from Libya. International Journal of Medical, Health, Pharmaceutical and Biomedical Engineering, 7 (12). pp. 703-705. English
spellingShingle Kammon, Abdulwahab
Tan, Sheau Wei
Omar, Abdul Rahman
Dayhum, Abdunaser
Eldghayes, Ibrahim
Sharif, Monier
Molecular detection and chracterization of infectious bronchitis virus from Libya.
title Molecular detection and chracterization of infectious bronchitis virus from Libya.
title_full Molecular detection and chracterization of infectious bronchitis virus from Libya.
title_fullStr Molecular detection and chracterization of infectious bronchitis virus from Libya.
title_full_unstemmed Molecular detection and chracterization of infectious bronchitis virus from Libya.
title_short Molecular detection and chracterization of infectious bronchitis virus from Libya.
title_sort molecular detection and chracterization of infectious bronchitis virus from libya.
url http://psasir.upm.edu.my/id/eprint/29928/
http://psasir.upm.edu.my/id/eprint/29928/1/Molecular%20detection%20and%20chracterization%20of%20infectious%20bronchitis%20virus%20from%20Libya.pdf