Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach
This study sought to isolate and characterize lactic acid bacteria (LAB) from stomach of adult snakehead fish, Channa striatus, to be used as probiotics for freshwater fish. A total of 13 strains were isolated from the stomach of 10 fish, and 4 of these belonged to LAB. Strain LAB-3 showing highest...
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29916/ http://psasir.upm.edu.my/id/eprint/29916/1/Characterization%20of%20a%20probiotic%20Lactobacillus%20fermentum%20isolated%20from%20snakehead.pdf |
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| author | Allameh, Sayed K. Md. Yusoff, Fatimah Mohd Daud, Hassan Ringo, Einar Ideris, Aini Saad, Che Roos |
| author_facet | Allameh, Sayed K. Md. Yusoff, Fatimah Mohd Daud, Hassan Ringo, Einar Ideris, Aini Saad, Che Roos |
| author_sort | Allameh, Sayed K. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study sought to isolate and characterize lactic acid bacteria (LAB) from stomach of adult snakehead fish, Channa striatus, to be used as probiotics for freshwater fish. A total of 13 strains were isolated from the stomach of 10 fish, and 4 of these belonged to LAB. Strain LAB-3 showing highest in vitro growth inhibition of Aeromonas hydrophila in a disk diffusion test was identified as Lactobacillus fermentum by conventional and molecular techniques and evaluated in vitro through various tests. The bacterium could grow at pH 3–8; but the optimum growth was observed at pH 6. Moreover, LAB-3 grew at 0.15 and 0.3% bile salt concentrations, from 15 to 45 C, and at 4% NaCl. L. fermentum showed in vitro inhibitory activity against three fish pathogens, A. hydrophila, Pseudomonas aeruginosa, and Shewanella putrefaciens, tested by disk diffusion and well diffusion methods. Antibiotic sensitivity tests indicated that L. fermentum was resistant to streptomycin, gentamycin, and kanamycin, intermediate to tetracycline, but sensitive to chloramphenicol, amoxicillin, and ampicillin. Challenge test by using A. hydrophila showed that survival of snakehead was significantly (P < 0.05) improved when 2 × 106 LAB-3/g was supplemented to the diet. Therefore, this study suggests that L. fermentum might be a promising probiotic in snakehead aquaculture. |
| first_indexed | 2025-11-15T09:04:12Z |
| format | Article |
| id | upm-29916 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:04:12Z |
| publishDate | 2013 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-299162015-09-08T07:02:30Z http://psasir.upm.edu.my/id/eprint/29916/ Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach Allameh, Sayed K. Md. Yusoff, Fatimah Mohd Daud, Hassan Ringo, Einar Ideris, Aini Saad, Che Roos This study sought to isolate and characterize lactic acid bacteria (LAB) from stomach of adult snakehead fish, Channa striatus, to be used as probiotics for freshwater fish. A total of 13 strains were isolated from the stomach of 10 fish, and 4 of these belonged to LAB. Strain LAB-3 showing highest in vitro growth inhibition of Aeromonas hydrophila in a disk diffusion test was identified as Lactobacillus fermentum by conventional and molecular techniques and evaluated in vitro through various tests. The bacterium could grow at pH 3–8; but the optimum growth was observed at pH 6. Moreover, LAB-3 grew at 0.15 and 0.3% bile salt concentrations, from 15 to 45 C, and at 4% NaCl. L. fermentum showed in vitro inhibitory activity against three fish pathogens, A. hydrophila, Pseudomonas aeruginosa, and Shewanella putrefaciens, tested by disk diffusion and well diffusion methods. Antibiotic sensitivity tests indicated that L. fermentum was resistant to streptomycin, gentamycin, and kanamycin, intermediate to tetracycline, but sensitive to chloramphenicol, amoxicillin, and ampicillin. Challenge test by using A. hydrophila showed that survival of snakehead was significantly (P < 0.05) improved when 2 × 106 LAB-3/g was supplemented to the diet. Therefore, this study suggests that L. fermentum might be a promising probiotic in snakehead aquaculture. Wiley-Blackwell 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29916/1/Characterization%20of%20a%20probiotic%20Lactobacillus%20fermentum%20isolated%20from%20snakehead.pdf Allameh, Sayed K. and Md. Yusoff, Fatimah and Mohd Daud, Hassan and Ringo, Einar and Ideris, Aini and Saad, Che Roos (2013) Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach. Journal of the World Aquaculture Society, 44 (6). pp. 835-844. ISSN 0893-8849; ESSN: 1749-7345 10.1111/jwas.12075 English |
| spellingShingle | Allameh, Sayed K. Md. Yusoff, Fatimah Mohd Daud, Hassan Ringo, Einar Ideris, Aini Saad, Che Roos Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title | Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title_full | Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title_fullStr | Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title_full_unstemmed | Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title_short | Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| title_sort | characterization of a probiotic lactobacillus fermentum isolated from snakehead, channa striatus, stomach |
| url | http://psasir.upm.edu.my/id/eprint/29916/ http://psasir.upm.edu.my/id/eprint/29916/ http://psasir.upm.edu.my/id/eprint/29916/1/Characterization%20of%20a%20probiotic%20Lactobacillus%20fermentum%20isolated%20from%20snakehead.pdf |