First molecular characterization of Giardia duodenalis from goats in Malaysia
In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29914/ http://psasir.upm.edu.my/id/eprint/29914/1/First%20molecular%20characterization%20of%20Giardia%20duodenalis%20from%20goats%20in%20Malaysia.pdf |
| _version_ | 1848846531103293440 |
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| author | Lim, Yvonne A. L. Mahdy, Mohammed A. K. Tan, Tiong Kai Goh, Xiang Ting Jex, Aaron R. Nolan, Matthew J. Kumar Sharma, Reuben Sunil Gasser, Robin B. |
| author_facet | Lim, Yvonne A. L. Mahdy, Mohammed A. K. Tan, Tiong Kai Goh, Xiang Ting Jex, Aaron R. Nolan, Matthew J. Kumar Sharma, Reuben Sunil Gasser, Robin B. |
| author_sort | Lim, Yvonne A. L. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the ‘zoonotic’ assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia. |
| first_indexed | 2025-11-15T09:04:11Z |
| format | Article |
| id | upm-29914 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:04:11Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-299142015-10-01T03:51:49Z http://psasir.upm.edu.my/id/eprint/29914/ First molecular characterization of Giardia duodenalis from goats in Malaysia Lim, Yvonne A. L. Mahdy, Mohammed A. K. Tan, Tiong Kai Goh, Xiang Ting Jex, Aaron R. Nolan, Matthew J. Kumar Sharma, Reuben Sunil Gasser, Robin B. In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the ‘zoonotic’ assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia. Elsevier 2013-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29914/1/First%20molecular%20characterization%20of%20Giardia%20duodenalis%20from%20goats%20in%20Malaysia.pdf Lim, Yvonne A. L. and Mahdy, Mohammed A. K. and Tan, Tiong Kai and Goh, Xiang Ting and Jex, Aaron R. and Nolan, Matthew J. and Kumar Sharma, Reuben Sunil and Gasser, Robin B. (2013) First molecular characterization of Giardia duodenalis from goats in Malaysia. Molecular and Cellular Probes, 27 (1). pp. 28-31. ISSN 0890-8508; ESSN: 1096-1194 10.1016/j.mcp.2012.08.006 English |
| spellingShingle | Lim, Yvonne A. L. Mahdy, Mohammed A. K. Tan, Tiong Kai Goh, Xiang Ting Jex, Aaron R. Nolan, Matthew J. Kumar Sharma, Reuben Sunil Gasser, Robin B. First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title | First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title_full | First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title_fullStr | First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title_full_unstemmed | First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title_short | First molecular characterization of Giardia duodenalis from goats in Malaysia |
| title_sort | first molecular characterization of giardia duodenalis from goats in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/29914/ http://psasir.upm.edu.my/id/eprint/29914/ http://psasir.upm.edu.my/id/eprint/29914/1/First%20molecular%20characterization%20of%20Giardia%20duodenalis%20from%20goats%20in%20Malaysia.pdf |