Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon

.Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of...

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Main Authors: Pourgholam, R., Mohd. Daud, Hassan, Kakoolaki, S., Khoshbavar Rostami, H. A., Mokarrami Rostami, A., Pourgholam, M. A.
Format: Article
Language:English
Published: Iranian Fisheries Research Organization 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29890/
http://psasir.upm.edu.my/id/eprint/29890/1/Some%20hematological%20and%20biochemical%20changes%20in%20blood%20serum%20of%20Grass%20carp.pdf
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author Pourgholam, R.
Mohd. Daud, Hassan
Kakoolaki, S.
Khoshbavar Rostami, H. A.
Mokarrami Rostami, A.
Pourgholam, M. A.
author_facet Pourgholam, R.
Mohd. Daud, Hassan
Kakoolaki, S.
Khoshbavar Rostami, H. A.
Mokarrami Rostami, A.
Pourgholam, M. A.
author_sort Pourgholam, R.
building UPM Institutional Repository
collection Online Access
description .Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of Grass Carp (Ctenopharyngodon idella).This experiment was carried out in three groups. The first group was vaccinated and exposed to diazinon (group A) while the second group was vaccinated and bathed with PBS bath (group B). The remaining fish were used as unvaccinated fish and were kept in clean water separately (group C). Diazinon was applied at concentrations of 2 mg/L for 12 hours since the experiments were initiated. The experimental groups(A and B) showed significantly lower values (p < 0.05) of erythrocyte count, haemoglobin content, haematocrit, leucocytes, Lymphocyte, myelocyte and monocyte, as well as in alkaline phosphatases, alanine aminotransferase, aspartate aminotransferase and, lactate dehydrogenase compared to the control group(C). Values of MCV, MCH and MCHC of experimental species (A and B) were compared to the control group(C). The results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes. Changes in values of both erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be referred to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish
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language English
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publisher Iranian Fisheries Research Organization
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spelling upm-298902015-10-28T06:05:27Z http://psasir.upm.edu.my/id/eprint/29890/ Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon Pourgholam, R. Mohd. Daud, Hassan Kakoolaki, S. Khoshbavar Rostami, H. A. Mokarrami Rostami, A. Pourgholam, M. A. .Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of Grass Carp (Ctenopharyngodon idella).This experiment was carried out in three groups. The first group was vaccinated and exposed to diazinon (group A) while the second group was vaccinated and bathed with PBS bath (group B). The remaining fish were used as unvaccinated fish and were kept in clean water separately (group C). Diazinon was applied at concentrations of 2 mg/L for 12 hours since the experiments were initiated. The experimental groups(A and B) showed significantly lower values (p < 0.05) of erythrocyte count, haemoglobin content, haematocrit, leucocytes, Lymphocyte, myelocyte and monocyte, as well as in alkaline phosphatases, alanine aminotransferase, aspartate aminotransferase and, lactate dehydrogenase compared to the control group(C). Values of MCV, MCH and MCHC of experimental species (A and B) were compared to the control group(C). The results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes. Changes in values of both erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be referred to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish Iranian Fisheries Research Organization 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29890/1/Some%20hematological%20and%20biochemical%20changes%20in%20blood%20serum%20of%20Grass%20carp.pdf Pourgholam, R. and Mohd. Daud, Hassan and Kakoolaki, S. and Khoshbavar Rostami, H. A. and Mokarrami Rostami, A. and Pourgholam, M. A. (2013) Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon. Iranian Journal of Fisheries Sciences, 12 (1). pp. 12-23. ISSN 1562-2916 http://www.jifro.ir/browse.php?a_id=868&sid=1&slc_lang=en
spellingShingle Pourgholam, R.
Mohd. Daud, Hassan
Kakoolaki, S.
Khoshbavar Rostami, H. A.
Mokarrami Rostami, A.
Pourgholam, M. A.
Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title_full Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title_fullStr Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title_full_unstemmed Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title_short Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon
title_sort some hematological and biochemical changes in blood serum of grass carp (ctenopharyngodon idella) vaccinated with aeromonas hydrophila following exposure to sublethal concentration of diazinon
url http://psasir.upm.edu.my/id/eprint/29890/
http://psasir.upm.edu.my/id/eprint/29890/
http://psasir.upm.edu.my/id/eprint/29890/1/Some%20hematological%20and%20biochemical%20changes%20in%20blood%20serum%20of%20Grass%20carp.pdf