Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study
Newcastle disease virus (NDV) strain AF2240 is a viscerotropic velogenic strain that is used as a vaccine challenge virus in Malaysia. The identification of the full length genome will be a crucial platform for further studies of this isolate. In this study, we fully sequenced the genome of a deriva...
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| Format: | Article |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29879/ |
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| author | Murulitharan, Kavitha Yusoff, Khatijah Omar, Abdul Rahman Molouki, Aidin |
| author_facet | Murulitharan, Kavitha Yusoff, Khatijah Omar, Abdul Rahman Molouki, Aidin |
| author_sort | Murulitharan, Kavitha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus (NDV) strain AF2240 is a viscerotropic velogenic strain that is used as a vaccine challenge virus in Malaysia. The identification of the full length genome will be a crucial platform for further studies of this isolate. In this study, we fully sequenced the genome of a derivative of this strain named AF2240-I. The 15,192 nt long genome contains a 55-nt leader sequence at the 3′ whereas the trailer region consists of 114 nt at the 5′. The intergenic sequences between the NP-P, P-M, M-F, F-HN, and HN-L genes comprise 1, 1, 1, 31, and 47 nt, respectively. The acknowledged cleavage site of fusion protein showed amino acid sequence of 112-R-R-Q-K-R-F-117, which corresponds to those of virulent NDV strains. Phylogenetic analysis of the whole virus genome shows that the strain AF2240-I belongs to genotype VIII and is more closely related to velogenic strains QH1, QH4, Fontana, Largo, and Italienas compared to other strains of NDV. Differences are noticed in the hemagglutinin-neuraminidase (HN) and matrix (M) gene between AF2240 and its derivative AF2240-I. This is the first report of a complete genome sequence of an NDV strain isolated in Malaysia. |
| first_indexed | 2025-11-15T09:04:02Z |
| format | Article |
| id | upm-29879 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:04:02Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-298792016-01-20T02:24:37Z http://psasir.upm.edu.my/id/eprint/29879/ Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study Murulitharan, Kavitha Yusoff, Khatijah Omar, Abdul Rahman Molouki, Aidin Newcastle disease virus (NDV) strain AF2240 is a viscerotropic velogenic strain that is used as a vaccine challenge virus in Malaysia. The identification of the full length genome will be a crucial platform for further studies of this isolate. In this study, we fully sequenced the genome of a derivative of this strain named AF2240-I. The 15,192 nt long genome contains a 55-nt leader sequence at the 3′ whereas the trailer region consists of 114 nt at the 5′. The intergenic sequences between the NP-P, P-M, M-F, F-HN, and HN-L genes comprise 1, 1, 1, 31, and 47 nt, respectively. The acknowledged cleavage site of fusion protein showed amino acid sequence of 112-R-R-Q-K-R-F-117, which corresponds to those of virulent NDV strains. Phylogenetic analysis of the whole virus genome shows that the strain AF2240-I belongs to genotype VIII and is more closely related to velogenic strains QH1, QH4, Fontana, Largo, and Italienas compared to other strains of NDV. Differences are noticed in the hemagglutinin-neuraminidase (HN) and matrix (M) gene between AF2240 and its derivative AF2240-I. This is the first report of a complete genome sequence of an NDV strain isolated in Malaysia. Springer 2013-01-10 Article PeerReviewed Murulitharan, Kavitha and Yusoff, Khatijah and Omar, Abdul Rahman and Molouki, Aidin (2013) Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study. Virus Genes, 46 (3). pp. 431-440. ISSN 0920-8569; ESSN: 1572-994X http://link.springer.com/article/10.1007%2Fs11262-012-0874-y 10.1007/s11262-012-0874-y |
| spellingShingle | Murulitharan, Kavitha Yusoff, Khatijah Omar, Abdul Rahman Molouki, Aidin Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title | Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title_full | Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title_fullStr | Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title_full_unstemmed | Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title_short | Characterization of Malaysian velogenic NDV strain AF2240-I genomic sequence: a comparative study |
| title_sort | characterization of malaysian velogenic ndv strain af2240-i genomic sequence: a comparative study |
| url | http://psasir.upm.edu.my/id/eprint/29879/ http://psasir.upm.edu.my/id/eprint/29879/ http://psasir.upm.edu.my/id/eprint/29879/ |