Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis.
The present study was carried out to elucidate the chemopreventive potential of methanol extract of Dicranopteris linearis (MEDL) in a two-stage mouse skin carcinogenesis model due to the interrelated inflammation, oxidative stress and tumor promotion pathways. MEDL was prepared in a dose range of 3...
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| Format: | Article |
| Language: | English |
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Academic Journals
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29825/ http://psasir.upm.edu.my/id/eprint/29825/1/Chemopreventive%20potential%20of%20methanol%20extract%20of%20Dicranopteris%20linearis%20leaf%20on%20DMBA.pdf |
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| author | R., Rodzi Cheah, Y. L. Ooi, K. K. Othman, Fezah Mohtarrudin, Norhafizah Md Tohid, Siti Farah Suhaili, Zarizal Zakaria, Zainul Amiruddin |
| author_facet | R., Rodzi Cheah, Y. L. Ooi, K. K. Othman, Fezah Mohtarrudin, Norhafizah Md Tohid, Siti Farah Suhaili, Zarizal Zakaria, Zainul Amiruddin |
| author_sort | R., Rodzi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study was carried out to elucidate the chemopreventive potential of methanol extract of Dicranopteris linearis (MEDL) in a two-stage mouse skin carcinogenesis model due to the interrelated inflammation, oxidative stress and tumor promotion pathways. MEDL was prepared in a dose range of 30 to 300 mg/kg body weight. A total of 48 imprinting control region (ICR) female mice (6 to 8 weeks old) were randomly assorted into six groups. To induce skin tumor formation, a single topical application of
7,12-dimethylbenz[a]anthracene (DMBA) at 100 μg/100 μl was applied to the shaved dorsal region of mice, followed by repetitive administration of 1% croton oil, twice weekly for 15 weeks. Topical application of MEDL, 30 min prior to the croton oil application significantly reduced the tumor incidence to 12.5% in 300 mg/kg MEDL-treated group as compared to 87.5% in carcinogen control. The latency
period of tumor formation was increased from sixth week in the carcinogen control to ninth and fifteenth weeks in 100 and 300 mg/kg MEDL-treated groups, respectively. The tumor burden of MEDL-treated groups (30, 100, and 300 mg/kg) were significantly lessen (5.67 ± 1.28, 5.00 ± 1.13, and 1.00 ± 0.13), as compared to carcinogen control (7.86 ± 2.37). The tumor volume was also significantly reduced from 9.00 ± 2.27 mm3 in carcinogen control to 3.70 ± 0.96, 2.39 ± 0.54 and 0.26 ± 0.03 mm3 in 30, 100 and 300 mg/kg MEDL-treated groups, respectively. In conclusion, the MEDL exhibited anti-carcinogenic effect in a dose-dependent manner, indicating its chemopreventive potential, which worth further study.
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| first_indexed | 2025-11-15T09:03:48Z |
| format | Article |
| id | upm-29825 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:03:48Z |
| publishDate | 2013 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-298252015-10-29T08:17:17Z http://psasir.upm.edu.my/id/eprint/29825/ Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis. R., Rodzi Cheah, Y. L. Ooi, K. K. Othman, Fezah Mohtarrudin, Norhafizah Md Tohid, Siti Farah Suhaili, Zarizal Zakaria, Zainul Amiruddin The present study was carried out to elucidate the chemopreventive potential of methanol extract of Dicranopteris linearis (MEDL) in a two-stage mouse skin carcinogenesis model due to the interrelated inflammation, oxidative stress and tumor promotion pathways. MEDL was prepared in a dose range of 30 to 300 mg/kg body weight. A total of 48 imprinting control region (ICR) female mice (6 to 8 weeks old) were randomly assorted into six groups. To induce skin tumor formation, a single topical application of 7,12-dimethylbenz[a]anthracene (DMBA) at 100 μg/100 μl was applied to the shaved dorsal region of mice, followed by repetitive administration of 1% croton oil, twice weekly for 15 weeks. Topical application of MEDL, 30 min prior to the croton oil application significantly reduced the tumor incidence to 12.5% in 300 mg/kg MEDL-treated group as compared to 87.5% in carcinogen control. The latency period of tumor formation was increased from sixth week in the carcinogen control to ninth and fifteenth weeks in 100 and 300 mg/kg MEDL-treated groups, respectively. The tumor burden of MEDL-treated groups (30, 100, and 300 mg/kg) were significantly lessen (5.67 ± 1.28, 5.00 ± 1.13, and 1.00 ± 0.13), as compared to carcinogen control (7.86 ± 2.37). The tumor volume was also significantly reduced from 9.00 ± 2.27 mm3 in carcinogen control to 3.70 ± 0.96, 2.39 ± 0.54 and 0.26 ± 0.03 mm3 in 30, 100 and 300 mg/kg MEDL-treated groups, respectively. In conclusion, the MEDL exhibited anti-carcinogenic effect in a dose-dependent manner, indicating its chemopreventive potential, which worth further study. Academic Journals 2013-09-22 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29825/1/Chemopreventive%20potential%20of%20methanol%20extract%20of%20Dicranopteris%20linearis%20leaf%20on%20DMBA.pdf R., Rodzi and Cheah, Y. L. and Ooi, K. K. and Othman, Fezah and Mohtarrudin, Norhafizah and Md Tohid, Siti Farah and Suhaili, Zarizal and Zakaria, Zainul Amiruddin (2013) Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis. African Journal of Pharmacy and Pharmacology, 7 (35). pp. 2484-2498. ISSN 1996-0816 http://www.academicjournals.org/journal/AJPP/article-stat/250B79640539 10.5897/AJPP2013.3575 |
| spellingShingle | R., Rodzi Cheah, Y. L. Ooi, K. K. Othman, Fezah Mohtarrudin, Norhafizah Md Tohid, Siti Farah Suhaili, Zarizal Zakaria, Zainul Amiruddin Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis. |
| title | Chemopreventive potential of methanol extract of
Dicranopteris linearis leaf on DMBA/croton oil-induced
mouse skin carcinogenesis.
|
| title_full | Chemopreventive potential of methanol extract of
Dicranopteris linearis leaf on DMBA/croton oil-induced
mouse skin carcinogenesis.
|
| title_fullStr | Chemopreventive potential of methanol extract of
Dicranopteris linearis leaf on DMBA/croton oil-induced
mouse skin carcinogenesis.
|
| title_full_unstemmed | Chemopreventive potential of methanol extract of
Dicranopteris linearis leaf on DMBA/croton oil-induced
mouse skin carcinogenesis.
|
| title_short | Chemopreventive potential of methanol extract of
Dicranopteris linearis leaf on DMBA/croton oil-induced
mouse skin carcinogenesis.
|
| title_sort | chemopreventive potential of methanol extract of
dicranopteris linearis leaf on dmba/croton oil-induced
mouse skin carcinogenesis. |
| url | http://psasir.upm.edu.my/id/eprint/29825/ http://psasir.upm.edu.my/id/eprint/29825/ http://psasir.upm.edu.my/id/eprint/29825/ http://psasir.upm.edu.my/id/eprint/29825/1/Chemopreventive%20potential%20of%20methanol%20extract%20of%20Dicranopteris%20linearis%20leaf%20on%20DMBA.pdf |